Giemsa solution is commonly used for hematology, histology, and parasitology labs. Peripheral blood smears and bone marrow smears are routinely stained in hematology labs. It is always preferred to use fresh peripheral blood smears or bone marrow smears when possible. The colors are more vibrant when the peripheral blood smears are stained fresh.
48900
Giemsa Stain, Modified Solution
(for the staining (of cellular blood components and blood parasites))
Synonym(s):
Azure eosin methylene blue, Giemsa solution
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100 ML
$76.80
500 ML
$138.00
1 L
$210.00
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41116121
PubChem Substance ID:
NACRES:
NA.25
Recommended Products
type
(for research use only)
Quality Level
shelf life
limited shelf life
technique(s)
microbe id | staining: suitable
suitability
Chlamydia spp.
Histoplasma spp.
Plasmodium spp.
Trichomonas spp.
bacteria
protozoa
spirochetes
SMILES string
[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key
NALREUIWICQLPS-UHFFFAOYSA-N
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Application
Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.
Caution
store at 20°C
Principle
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
Physical form
Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)
Reconstitution
Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
Target Organs
Eyes
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Flash Point(F)
53.6 °F
Flash Point(C)
12 °C
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Hi does any lab use this stain for microscopy ( haematology) ? How is it working for you on fresh peripheral blood films ?
1 answer-
Helpful?
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Is there a white paper available for buffer selection for Giemsa staining?
1 answer-
Generally, no white papers are available. Giemsa formulations vary in pH, typically ranging between pH 6.4 to 6.9. Quality standards for stained slides differ across institutions and individuals, with varying preferences for pH buffers.
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