69522
T7•Tag® Monoclonal Antibody
Synonym(s):
T7 Tag Antibody
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About This Item
UNSPSC Code:
12352203
Recommended Products
clone
monoclonal
species reactivity
mouse
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG2b
shipped in
dry ice
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN® and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select® Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
Specificity
T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Cross-reactivity: Negligible with bacterial, insect or mammalian cell lysates
Sensitivity: 1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
Working dilution- 1:5,000–1:10,000
Cross-reactivity: Negligible with bacterial, insect or mammalian cell lysates
Sensitivity: 1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
Working dilution- 1:5,000–1:10,000
Packaging
Plastic ampoule
Warning
Multiple Toxicity Values, refer to MSDS
Physical form
Stabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Other Notes
- Krek, W., et al. 1994. Cell 78,, 161.
- Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.
- Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PEPscreen is a registered trademark of Sigma-Aldrich Co. LLC
T7-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany
T7SELECT is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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