07-937

Anti-HDAC7 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): histone deacetylase 7, histone deacetylase 7A
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): western blot: suitable
• NCBI accession no.: NP_001091886.1
• UniProt accession no.: Q8WUI4
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HDAC7(51564)

Description

General description

Histone deacetylases (HDAC) are enzymes that regulates transcription by selectively deacetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of the HDAC family have been identified, divided in to two classes. Class I includes HDAC-1, 2, 3 and 8 while Class II includes HDAC-4, 5, 6, and 7. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a C-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional co-repressor complexes that can be recruited to specific promoter regions via interactions with transcription factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones, resulting in a tighter DNA-histone interaction. This compaction of chromatin leads to the repression of transcription of DNA in that region.

Specificity

Not tested with other species.

This antibody recognizes human HDAC7. Does not cross-react with HDAC1, HDAC4, or HDAC8.

Immunogen

Epitope: a.a.164-182

Synthetic peptide corresponding to amino acids 164-182 of human HDAC7 [(C)KPKKSLERRKNPLLRKESA].

Application

Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer

Research Sub Category
Histones

Use Anti-HDAC7 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect HDAC7 also known as histone deacetylase 7, histone deacetylase 7A.

Western Blotting:
A previous lot of this antibody was shown to recognize recombinant HDAC7 but not recombinant HDAC1, HDAC4, or HDAC8.

Western Blotting:
A previous lot of this antibody was shown to recognize endogenous HDAC7 in HEK293T cells.

Quality

Routinely evaluated by Western blotting using recombinant HDAC7 protein.

Target description

100 kDa

Physical form

Affinity purified

Purified Rabbit polyclonal in buffer containing 20 mM PBS and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Recombinant HDAC7 protein (Cat. #14-832).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable