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MAK090

Sigma-Aldrich

γ-Glutamyltransferase (GGT) Activity Fluorometric Assay Kit

sufficient for 100 fluorometric tests

Synonym(s):

Gamma Glutamyltransferase Activity Fluorometric Assay Kit

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About This Item

EC Number:
200-664-3
UNSPSC Code:
12352200

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... GGT1(2678) , GGT2(728441) , GGT5(2687) , GGT6(124975) , GGT7(2686)
mouse ... GGT1(14598) , GGT5(23887) , GGT6(71522) , GGT7(207182)
rat ... GGT1(116568) , GGT5(296566) , GGT6(408206) , GGT7(156275)

General description

γ-Glutamyltransferase (GGT, EC 2.3.2.2) is a membrane-bound protein that catalyzes the transfer of γ-glutamyl moieties to acceptor molecules such as amino acids or peptides. GGT plays a key role in the γ-glutamyl cycle, a critical pathway for glutathione homeostasis as well as the detoxification of xenobiotics. Serum GGT levels can be elevated in many pathophysiological conditions such as cardiovascular disease, chronic liver disease, and metabolic syndrome. Elevated serum levels of GGT can also be indicative of oxidative stress.

Application

γ-Glutamyltransferase (GGT) Activity Fluorometric Assay Kit has been used to analyze serum GGT activity.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of gamma-glutamyl transferase (GGT) in a variety of samples including tissue, cells and serum

Principle

The GGT Activity Fluorometric Assay kit provides a simple and direct procedure for measuring GGT activity in a variety of samples. GGT activity is determined by a coupled enzyme assay, in which the GGT transfers the γ-glutamyl group from quenched substrate L-γ-Glutamyl-7-amido-4-methylcoumarin (L--Glutamyl-AMC), liberating the fluorescent group, 7-Amino-4-Methyl Coumarin (AMC) (λex = 365 nm/λem = 460 nm) proportional to the GGT activity present. One unit of GGT is the amount of enzyme that will generate 1.0 μmole of AMC per minute at 37 °C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

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Ruben Weckx et al.
Scientific reports, 12(1), 10591-10591 (2022-06-23)
In septic mice, 3-hydroxybutyrate-sodium-salt has shown to partially prevent sepsis-induced muscle weakness. Although effective, the excessive sodium load was toxic. We here investigated whether ketone ester 3-hydroxybutyl-3-hydroxybutanoate (3HHB) was a safer alternative. In a mouse model of abdominal sepsis, the
Jia-Ru Wei et al.
Aging, 12(13), 13220-13233 (2020-07-09)
Gamma-glutamyltransferase 5 (GGT5) is a member of the gamma-glutamyl transpeptidase gene family with the capacity of cleaving the gamma-glutamyl moiety of glutathione, but its role in cancer progression has never been revealed. In this study, we found that gene GGT5
An ultrasensitive bioluminogenic probe of ?-Glutamyltranspeptidase in vivo and in human serum for tumor diagnosis.
Li S, et al.
Biosensors And Bioelectronics, 98, 325-329 (2017)
Gamma-glutamyl transferases: A structural, mechanistic and physiological perspective.
Balakrishna S and Asmita A P
Frontiers in Biology, 9(1), 51-65 (2014)
A modified choline-deficient, ethionine-supplemented diet reduces morbidity and retains a liver progenitor cell response in mice.
Passman A M, et al.
Disease models & mechanisms, 8(12), 1635-1641 (2015)
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