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L0144

Sigma-Aldrich

Lucifer Yellow CH dipotassium salt

powder

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25 MG
€199.00
100 MG
€318.00

€199.00

Estimated to ship on27 March 2025

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25 MG
€199.00
100 MG
€318.00

About This Item

Empirical Formula (Hill Notation):
C13H9K2N5O9S2
CAS Number:
71206-95-6
Molecular Weight:
521.57
Beilstein:
5224942
MDL number:
MFCD00013445
UNSPSC Code:
12171500
PubChem Substance ID:
24896249
NACRES:
NA.77

€199.00

Estimated to ship on27 March 2025

Request a Bulk Order

form

powder

Quality Level

100

color

orange

fluorescence

λex 428 nm; λem 540 nm

storage temp.

room temp

SMILES string

[K+].[K+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

InChI key

HCWYSCVNSCIZCN-UHFFFAOYSA-L

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General description

Lucifer yellow CH is a fixable dye with an excitation and emission spectrum of 430 nm and 540 nm, respectively.[1]LuciferYellow CH (LYCH) is a small, hydrophilic molecule,[2] and highly fluorescent dye that is used for intracellular staining for the studies conducted on various types of cells.[3] It acts as a marker for the detection of well-established tight junctions[2] in smooth muscles, glia, and interstitial cells of Cajal (ICC).[3]

Application

Lucifer yellow CH dipotassium salt has been used:
  • to visualize cell morphology[1]
  • to assess intercellular communication activity through gap junction by scrape-loading assay[4][5]
  • as a component of the intracellular solution, which filled the dendrites for the visualization of neuronal morphology[6]
  • in the intracellular solution, to intracellularly label recorded neurons[7]

Features and Benefits

Highly fluorescent dye, useful in marking nerve cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SHBR8105
SHBR1397
SHBQ2310
SHBP0869
SHBN6250

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In vitro mesenchymal-epithelial transition in NIH3T3 fibroblasts results in onset of low-dose radiation hypersensitivity coupled with attenuated connexin-43 response
Mathur A, et al.
Biochimica et biophysica acta. General subjects, 1862(3), 414-426 (2018)
Shuaiwei Wang et al.
Mediators of inflammation, 2020, 8094347-8094347 (2020-12-10)
Connexin (Cx) family members form hemichannels (HCs) and gap junctions (GJs). Biological functions of Cx HCs have not been adequately characterized due to the inability to selectively target HCs or GJs. Recently, we developed a 6-mer peptide mimetic (P5) of
Zebrafish retinal slice preparation
Connaughton, VP
Methods in Cell Science : An Official Journal of the Society for In Vitro Biology, 25(1-2), 49-58 (2003)
In vitro and in vivo models of BBB to evaluate brain targeting drug delivery
Sharma B, et al.
Brain, 53-101 (2019)
Connexin-43 downregulation in G2/M phase enriched tumour cells causes extensive low-dose hyper-radiosensitivity (HRS) associated with mitochondrial apoptotic events
Ghosh S, et al.
Cancer letters, 363(1), 46-59 (2015)
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Questions

1–2 of 2 Questions  

  1. What is the dye purity for product L0144?

    1 answer

    1. At M-Clarity quality level 100, Product L0144 is evaluated based on appearance, infrared spectrum, solubility, and fluorescence specifications. Please refer to the Certificate of Analysis found on the DOCUMENTATION section of the Product Detail Page: https://www.sigmaaldrich.com/product/sigma/l0144#product-documentation

      Using the results on the specification sheet, dye content can be calculated from the testing performed to determine carbon and nitrogen levels.

      Helpful?

  2. What is the stability and shelf life for product L0144, lot SHBN6250 once it has been prepared in a solution?

    1 answer

    1. The product is soluble in water at a concentration of 1 mg/mL, producing a clear, yellow-orange colored solution. It is also soluble in water with heat at a concentration of 10 mg/mL, resulting in a slightly hazy orange colored solution. Additionally, a solution stability test revealed that a solution of L0144 in water at a concentration of 1.7 mg/mL, stored in the dark at room temperature for 32 days, showed no detectable change in the UV-VIS spectrum.

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