ABS986

Anti-PCTP Antibody

serum, from rabbit

• Synonym(s): Phosphatidylcholine transfer protein, PC-TP, START domain-containing protein 2, StARD2, StAR-related lipid transfer protein 2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, IP, WB
• Citations: 2

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_067036
• UniProt accession no.: Q9UKL6
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PCTP(58488)

Description

General description

Phosphatidylcholine transfer protein (PCTP), also known as PC-TP, START domain-containing protein 2, StARD2, StAR-related lipid transfer protein 2, and encoded by the gene symbol PCTP/ STARD2, belongs to the steroidogenic acute regulatory protein related transfer (START) domain superfamily of hydrophobic ligand-binding proteins. PCTP has been shown to play an important role in the transfer of phosphatidylcholine between membranes. The crystal structures of human PC-TP in complex with dilinoleoyl-PtdCho or palmitoyl-linoleoyl-PtdCho suggests that a single well-ordered PtdCho molecule occupies a centrally located tunnel. These structures have provided a basis for rationalizing the specificity of PC-TP for PtdCho and may identify common features used by START proteins to bind their hydrophobic ligands. Additionally, PCTP has been demonstrated to interact with ACOT13/THEM2. PCTP is located in the cytoplasm with highest expression in liver, placenta, testis, kidney and heart. Low levels of PCTP are expressed in brain and lung.

~33 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human PCTP.

Application

Anti-PCTP is an antibody against PCTP for use in WB, ICC, IP.

Western Blotting Analysis: A representative lot detected PCTP in cytosolic fractions isolated from the liver of a control subject, a patient with intrahepatic brown pigment stones, and a patient with obstructive cholestasis (Shoda, J., et al. (2001). Hepatology. 33(5):1194-1205).
Western Blotting Analysis: A representative lot detected PCTP in siRNA-transfected, serum-starved HEK 293E cells, Pctp+/+ liver lysates, Tsc2−/− MEFs, Liver homogenates from chow diet–fed Pctp+/+ (n = 4) and Pctp−/− (n = 4) mice (Ersoy, B.A., et al. (2013). Science Signaling. 286(6):1-11).
Immunocytochemistry Analysis: A representative lot detected PCTP in HEK293T cells (Kanno, K., et al. (2007). J. Biol. Chem. 282:30728-30736).
Immunoprecipitation Analysis: A representative lot immunoprecipitated PCTP in Pctp+/+ liver lysates (Ersoy, B.A., et al. (2013). Science Signaling. 286(6):1-11).

Analysis Note

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PCTP in 5 µg of HepG2 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1