ABE419
Anti-Histone H3.3 Antibody, K27M mutant
from rabbit, purified by affinity chromatography
Synonym(s):
Histone H3.1, Histone H3.3
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100 μG
€557.00
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
technique(s)
ChIP: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
mutation (Lys27Met)
Gene Information
human ... H3F3B(3021)
General description
Histone H3.3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3.3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.
Specificity
This antibody recognizes Histone H3.3 with K27M mutation.
Immunogen
Epitope: Histsone H3 sequence surrounding K27M mutation
KLH-conjugated linear peptide corresponding to sequence near the N-terminus of human Histone H3.3 with K27M mutation.
Application
- Peptide Inhibition Assay (PIA): Target band detection inlysate from HEK-293 cells transfected with Histone H3.3 K27M mutant wasprevented by pre-blocking of a representative lot with the immunogen HistoneH3.3 K27M mutant peptide, but not the corresponding unmodified Histone H3.3 peptide.
- Immunohistochemistry (IHC): A representative lot of this antibodydetected Histone 3.3 K27M in pediatric glioblastoma tissue sections. (Venneti,S., et al. (2014). Acta Neuropathol 128(5); 743-753).
Anti-Histone H3.3 Antibody, K27M mutant, is validated for use in western blotting (WB) & Chromatin immunoprecipitation (ChIP).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Quality
Evaluated by Western Blotting in HEK-293 cellstransfected with Histone H3.3 K27M.
Western Blotting Analysis (WB): A 1:2,000 dilution of a representative lot of thisantibody detected Histone H3.3 K27M in HEK-293 cells transfected with HistoneH3.3 K27M mutant.
Western Blotting Analysis (WB): A 1:2,000 dilution of a representative lot of thisantibody detected Histone H3.3 K27M in HEK-293 cells transfected with HistoneH3.3 K27M mutant.
Target description
~17 kDa observed
Physical form
Immunogen Affinity Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Lysates from MEF transfectants expressing K27M (positive) or wildtype (negative) FLAG-HA-tagged histone H3.3.
Lysates from MEF transfectants expressing K27M (positive) or wildtype (negative) FLAG-HA-tagged histone H3.3.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mélanie Pagès et al.
Brain pathology (Zurich, Switzerland), 28(1), 103-111 (2016-12-17)
Ganglioglioma (GG) is a grade I tumor characterized by alterations in the MAPK pathway, including BRAF V600E mutation. Recently, diffuse midline glioma with an H3 K27M mutation was added to the WHO 2016 classification as a new grade IV entity.
Thomas J Stone et al.
Acta neuropathologica, 135(1), 115-129 (2017-10-24)
Glioneuronal tumours are an important cause of treatment-resistant epilepsy. Subtypes of tumour are often poorly discriminated by histological features and may be difficult to diagnose due to a lack of robust diagnostic tools. This is illustrated by marked variability in
Hamid Nikbakht et al.
Nature communications, 7, 11185-11185 (2016-04-07)
Diffuse Intrinsic Pontine Gliomas (DIPGs) are deadly paediatric brain tumours where needle biopsies help guide diagnosis and targeted therapies. To address spatial heterogeneity, here we analyse 134 specimens from various neuroanatomical structures of whole autopsy brains from nine DIPG patients.
Abstracts from USCAP 2019: Neuropathology and Ophthalmic Pathology (1621-1666).
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 32(Suppl 2), 1-39 (2019-03-20)
Specific detection of methionine 27 mutation in histone 3 variants (H3K27M) in fixed tissue from high-grade astrocytomas.
Bechet, D; Gielen, GG; Korshunov, A; Pfister, SM; Rousso, C; Faury, D; Fiset et al.
Acta neuropathologica null
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