05-1572

Anti-MDC1 Antibody, clone P2B11

clone P2B11, from mouse

• Synonym(s): Nuclear factor with BRCT domains 1, homologue to Drosophila photoreceptor protein calphotin, mediator of DNA damage checkpoint 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: P2B11, monoclonal
• species reactivity: mouse
• species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology), bovine (based on 100% sequence homology), human (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001010833.2
• UniProt accession no.: Q5PSV9
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MDC1(9656) ; mouse ... Mdc1(240087)

Description

General description

MDC1 is a subunit of the cohesin complex and is an essential protein required for sister chromatid cohesion in mitosis and meiosis. Temperature-sensitive mutations of this protein have been associated with defects in sister chromatid cohesion, chromosome condensation, and chromosome segregation. Its expression is cell cycle regulated and peaks in S phase. Expression of MDC1 is required for the S- and G2/M-checkpoint-mediated cell cycle arrest in response to DNA damage. MDC1 has been shown to interact with H2A.X near sites of DNA double-strand breaks and recruit ATM kinase to the damaged region of DNA. Consistent with this role, MDC1 also appears to modulate the function of other proteins involved in the DNA damage checkpoint such as BRCA1 and Chk2.

Specificity

This antibody recognizes MDC1 at and around the N-terminus.

Immunogen

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.

Application

Anti-MDC1 Antibody, clone P2B11 is a Mouse Monoclonal Antibody for detection of MDC1 also known as Nuclear factor with BRCT domains 1 & has been validated in ICC & WB.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected MDC1 in NIH/3T3 cells.

Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Lou, Z., et al. (2003). Nature. 421(6926):957-961.)

Target description

~ 184 kDa There are 4 isoforms of human MDC1 because of splicing with molecular weights of approximately 227, 198, 196, and 117 kDa

Physical form

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) without 0.05% sodium azide.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable