Anti-Cytochrome c antibody detects endogenous levels of total Cytochrome c protein.
The cytochrome c gene is mapped to human chromosome 7p15.3. The encoded protein consists of two thioether present between heme and two cysteine residues included in a CXXCH motif (highly conserved). It is localized to mitochondrial intermembrane space.
Immunogen
The antiserum was produced against synthesized peptide derived from human Cytochrome c.
Immunogen Range: 10-59
Application
Anti-Cytochrome c, N-terminal antibody produced in rabbit has been used in western blot analysis.
Biochem/physiol Actions
Cytochrome c is a water-soluble intermediary of electron transport chain. It acts as an ROS (reactive oxygen species) scavenger, and involved in redox-mediated protein import and apoptosis. Cytochrome c has peroxidase activity, contributing towards intrinsic pathway of apoptosis.
Features and Benefits
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Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Effect of a K72A Mutation on the Structure, Stability, Dynamics, and Peroxidase Activity of Human Cytochrome c.
Nold SM
Biochemistry, 56(26), 3358-3368 (2017)
Evaluation of the adaptogenic potential exerted by ginsenosides Rb1 and Rg1 against oxidative stress-mediated neurotoxicity in an in vitro neuronal model.
Fernandez-Moriano C
PLoS ONE, 12(8), 1-22 (2017)
Enhancing the peroxidase activity of cytochrome c by mutation of residue 41: implications for the peroxidase mechanism and cytochrome c release.
Josephs TM
The Biochemical Journal, 458(2), 259-265 (2014)
Biosynthesis of Single Thioether c-Type Cytochromes Provides Insight into Mechanisms Intrinsic to Holocytochrome c Synthase (HCCS).
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