UHPLC Gradient Analysis of Boswellic Acids on Ascentis^® Express Phenyl-Hexyl

column

Ascentis Express Phenyl-Hexyl, 10 cm x 3.0 mm I.D., 2.7 μm particles (53345-U)

mobile phase

[A] 0.1% phosphoric acid; [B] acetonitrile

gradient

50% B for 0.5 min; to 100% B in 4.5 min; held at 100% B for 5 min

flow rate

0.6 mL/min

pressure

2390 psi (165 bar)

column temp.

35 °C

detector

UV, 210 nm

injection

2 μL

sample

6 Boswellic Acids 50μg/mL in 70:30, water:methanol