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RP-HPLC Analysis of Benzodiazepines on Ascentis® Express Biphenyl

RP-HPLC Analysis of Benzodiazepines on Ascentis® Express Biphenyl application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

Ascentis® Express Biphenyl Column

2.7 μm particle size, L × I.D. 10 cm × 3.0 mm

Standard

Produkt-Nr.
Beschreibung
Preisangaben

Oxazepam -Lösung

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Alprazolam -Lösung

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Clonazepam -Lösung

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Diazepam -Lösung

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Flunitrazepam -Lösung

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Lorazepam -Lösung

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

Nitrazepam -Lösung

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

Temazepam -Lösung

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express Biphenyl, 10 cm x 3.0 mm I.D., 2.7 μm particles (64066-U)

mobile phase

[A] Water; [B] acetonitrile; (70:30, A:B)

flow rate

1.0 mL/min

pressure

5294 psi (365 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

1.0 μL

sample

Benzodiazepine Multicomponent Mixture-8 diluted to 50ug/mL in 50:50 Water:Methanol

Beschreibung

Hinweis zur Analyse

This analysis demonstrates the applicability of the Ascentis Express Biphenyl stationary phase for the analysis of benzodiazepines, a group of psychoactive drugs that act as GABA agonists. The stationary phase provides alternative selectivity and improved resolution to existing phenyl chemistires.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany