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  • A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH.

A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH.

Journal of biochemical and biophysical methods (2006-12-16)
Joseph A Price
ZUSAMMENFASSUNG

Phospholipase A(2) is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Bromthymolblau, ACS reagent, Dye content 95 %
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