ZRB1564

Anti-CD24 Antibody, clone 3N22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Signal transducer CD24, Small cell lung carcinoma cluster 4 antigen
• NACRES: NA.43
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 3N22, monoclonal, recombinant monoclonal
• Application: FACS, WB, affinity binding assay
• Citations: -

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3N22, monoclonal, recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 8.08 kDa, observed mol wt ~38 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): affinity binding assay: suitable, flow cytometry: suitable, western blot: suitable
• isotype: IgG
• UniProt accession no.: P25063
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD24(100133941)

Beschreibung

General description

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Signal transducer CD24 (UniProt: P25063; also known as Small cell lung carcinoma cluster 4 antigen, CD24) is encoded by the CD24 (also known as CD24A) gene (Gene ID: 100133941) in human. CD24 is a GPI-anchor membrane glycoprotein protein that is expressed on hematopoietic cells including B cells, T cells, neutrophils, macrophages and in neuronal and ganglion cells. Its expression is also observed in many types of cancer cells. Its expression is reported to be higher levels in progenitor cells and metabolically active cells compared to terminally differentiated cells. In B cells its expression is lost when primary B-cells are induced to differentiate in antibody-forming cells. Deficiency of CD24 can result in a reduction in late pre-B and immature B-cell populations in the bone marrow. CD24 is extensively used as a marker for the differentiation of hematopoietic and neuronal cells. As a glycosyl phosphatidylinositol-anchor molecule, it mediates signal transduction by recruiting Src family protein tyrosine kinases via membrane rafts and also activates the mitogen-activated protein kinase pathway. CD24 is synthesized with a signal peptide (aa 1-26) and a propeptide (aa 60-80) that are cleaved off to produce the mature form (aa 27-59). Mutation is CD24 gene are linked to development of multiple sclerosis, a demyelinating disease of the central nervous system. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fang, X., et al. (2010). Cell. Mol. Immunol. 7(2); 100-103).

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human Signal transducer 24 (CD24).

Application

Anti-CD24, clone 3N22 ZooMAb, Cat. No. ZRB1564, is a recombinant Rabbit monoclonal antibody that specifically detects CD24 and is tested for use in Affinity Binding Assay, Flow Cytometry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD24 in lymphocytes, Jurkat, and Daudi cell lysates.

Flow Cytometry Analysis: 1 μg from a representative lot detected CD24 in one million Raji cells.

Affinity Binding Assay: A representative lot of this antibody bound CD24 peptide with a KD of 3.4 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Biochem/physiol Actions

Clone 3N22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Signal transducer 24 (CD24).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable