It is a good antibody for Western Blot for both specimen of human and mouse. But it often shows signals medium intensity, not strong enough.
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
0.2 mg | Warenkorb auf Verfügbarkeit prüfen | € 534,00 |
Über diesen Artikel
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
technique(s)
immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells, immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells, western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Der synthetische Anti-V5-Peptidantikörper vom Kaninchen reagiert mit V5-markierten rekombinanten Fusionsproteinen, die in transfizierten Säugetierzellen und aus der In-vitro-Translation exprimiert werden.
Application
- Immunzytochemie
- Immunpräzipitation
- Western Blot
Biochem/physiol Actions
Physical form
Preparation Note
Disclaimer
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| antibody form IgG fraction of antiserum | antibody form affinity isolated antibody | antibody form IgG fraction of antiserum | antibody form affinity isolated antibody |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| technique(s) immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells, western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells, immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells | technique(s) immunoprecipitation (IP): 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates (tagged fusion proteins from transfected mammalian cell lysates), indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells, western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates | technique(s) immunoprecipitation (IP): 10 μg using mammalian cell extracts expressing VP16 fusion proteins, western blot: 1:1,000 using mammalian cell extracts expressing VP16 fusion proteins | technique(s) immunoprecipitation (IP): 5-10 μg using lysates of human Jurkat cells., western blot: 2.5-5 μg/mL using whole extracts of mouse A20 cells. |
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Lagerklasse
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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