T7201

Thrombin aus Rinderplasma

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

• Synonym(e): Faktor IIa
• CAS-Nummer: 9002-04-4
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.4.21.5 (BRENDA, IUBMB)
• MDL number: MFCD00166053
• Specific activity: ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

Eigenschaften

• form: lyophilized powder
• specific activity: ≥2,000 NIH units/mg protein (E1%/280 = 19.5)
• mol wt: heavy chain ~31 kDa, light chain ~6 kDa
• storage temp.: −20°C

Beschreibung

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Thrombin consists of A-chain and a catalytic B-chain with the catalytic pocket comprising of His57, Asp102, Ser195 and Ser214. It has a fibrinogen recognition site (FRS) and a heparin binding site (HBS) in the C-terminus. The main function of thrombin is cleavage of fibrinogen to fibrin. It coordinates the coagulation cascade and binds to diverse substrates. It coordinates with protease-activated receptors (PARs) to accomplish the physiological functions. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contribute to the progression of Parkinson′s disease. Altered thrombin levels modulates coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess a novel antiplatelet therapy in acute coronary syndromes.

Thrombin from bovine plasma has been used in the cleavage of the maltose binding protein tag in mini-calpain-5 fusion protein and in thrombin/hirudin spiking experiments in serum samples.

Biochem/physiol Actions

Serinprotease, die selektiv Arg-Gly-Bindungen in Fibrinogen spaltet, um Fibrin und Fibrinopeptide A und B zu bilden.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Preparation Note

Traditional thrombin products are activated with bovine brain, whereas T7201 is activated with bovine lung and does not contain any bovine brain products.

Analysis Note

Beim NIH-Assay werden 0,2 ml verdünntes Plasma (1:1 mit Kochsalzlösung) als Substrat und 0,1 ml der Thrombinprobe (stabilisiert in einer 1%igen gepufferten Albuminlösung) auf Basis einer modifizierten Biggs-Methode analysiert. Zur Bestimmung der Thrombinkonzentrationen werden nur Gerinnungszeiten im Bereich von 15 bis 25 Sekunden verwendet.

Die Aktivität wird in NIH-Einheiten ausgedrückt, die aus einem direkten Vergleich mit einem NIH-Thrombin-Referenzstandard (Charge K) gewonnen werden

Other Notes

Für weitere Informationen siehe thrombin at www.sigma-aldrich.com/enzymeexplorer.

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)