SRP2032

Topo I (C651-765) (C terminal domain) human

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

• Synonym(e): TOPI
• UNSPSC-Code: 12352202
• NACRES: NA.26

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in insect cells
• Assay: ≥80% (SDS-PAGE)
• Form: frozen liquid
• Mol-Gew.: ~15.2 kDa
• Verpackung: pkg of 5 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: 500 μg/mL
• Farbe: clear colorless
• NCBI-Hinterlegungsnummer: NM_003286
• UniProt-Hinterlegungsnummer: P11387
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... TOP1(7150)

Beschreibung

Biochem./physiol. Wirkung

Human DNA topoisomerase I catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, recombination, chromatin remodeling and other DNA or RNA templating activities. The C terminal domain of DNA topoisomerase I spanning from amino acids 713 to 765 is highly conserved and connected to the core domain by a poorly conserved linker domain (residues 636-713). An active site tyrosine has been characterized at position 723. Mutation of this tyrosine to phenylalanine at position 723 causes topo I to preferentially bind the supercoiled DNA rather than relaxed DNA in the mixture of supercoiled and relaxed DNA.

Physikalische Form

Clear and colorless frozen liquid solution

Angaben zur Herstellung

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable