SRP2020

TR, β 1 isoform human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonym(e): ERBA-beta, GRTH, NR1A2, THR1, THRB1
• UNSPSC-Code: 12352202
• NACRES: NA.26

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥70% (SDS-PAGE)
• Form: frozen liquid
• Mol-Gew.: ~53 kDa
• Verpackung: pkg of 10 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: 500 μg/mL
• Farbe: clear colorless
• NCBI-Hinterlegungsnummer: NM_000461
• UniProt-Hinterlegungsnummer: P10828
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... THRB(7068)

Beschreibung

Biochem./physiol. Wirkung

Nuclear receptors form the largest known family of transcription factors and have a crucial role in nearly all aspects of vertebrate development and adult physiology by transducing the effects of hormones into transcriptional responses. The family is defined by two domains: (a) the central, highly conserved, DNA-binding domain (DBD) of approximately 66 amino acids, and (b) the C-terminal, structurally conserved, ligand-binding domain (LBD) of approximately 250 amino acids. The amino-terminal regions are least conserved among nuclear receptor sequences. This domain is highly divergent between TR α and TR β isoforms, which suggests differential roles in transcriptional regulation. In addition, alternative splicing of the TR β gene generates two isoforms, TR β1 and TR β2 with completely different amino-terminal domains. Unliganded TR inhibits the formation of a functional pre-initiation complex, through direct interaction with TBP and transcription factor IIB. In addition, in the absence of ligand TR has been shown to repress transcription through recruitment of a corepressor complex, which also includes Sin3A and histone deacetylase. Ligand binding releases the corepressor complex and recruits a coactivator complex that includes multiple histone acetyltransferases, including a steroid receptor family coactivator, p300/CREB-binding protein–associated factor (PCAF), and CREB binding protein (CBP).

Physikalische Form

Clear and colorless frozen liquid solution

Angaben zur Herstellung

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable