SAB4702000

Monoclonal Anti-CD34 PerCP-Cyanine5.5 produced in mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• Conjugate: conjugate
• Clone: 581, monoclonal
• Application: FACS
• Citations: -

Eigenschaften

• conjugate: conjugate
• Quality Segment: 100
• antibody product type: primary antibodies
• clone: 581, monoclonal
• form: buffered aqueous solution
• species reactivity: (Non-human primates), ( Human)
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• UniProt accession no.: P28906
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD34(947)

Beschreibung

General description

CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Biochem/physiol Actions

The mouse monoclonal antibody 581 reacts with an extracellular epitope of CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease.

Physical form

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable