SAB4200170

Anti-CPSF4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(e): Anti-CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR 4, Anti-CPSF30NS1 EFFECTOR DOMAIN-BINDING PROTEIN 1, Anti-NEB1
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~30 kDa
• Speziesreaktivität: human
• Verpackung: antibody small pack of 25 μL
• Konzentration: ~1.0 mg/mL
• Methode(n): western blot: 1-2 μg/mL using Whole extracts of HEK-293T cells overexpressing human CPSF4.
• UniProt-Hinterlegungsnummer: O95639
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CPSF4(10898) ; mouse ... CPSF4(54188) ; rat ... CPSF4(304277)

Beschreibung

Allgemeine Beschreibung

Cleavage polyadenylation specificity factor 4 (CPSF4), also called CPSF 30 is a component of the CPSF complex. It comprises of five CCCH zinc finger motifs (ZF1-ZF5). CPSF4 gene is mapped to human chromosome 7q22.1.

Spezifität

Anti-CPSF4 recognizes human CPSF4.

Anwendung

Anti-CPSF4 antibody produced in rabbit has been used in chromatin immunoprecipitation and immunoblotting.

Biochem./physiol. Wirkung

Cleavage polyadenylation specificity factor 4 (CPSF4) is a component of 3′-end mRNA processing machinery and binds to poly(U) sequence of RNA via the zinc finger motifs. These motifs are also involved in protein-protein interactions. CPSF4 along with other proteins primarily coordinate both cleavage and polyadenylation. Their interaction with the non-structural protein 1 (NS1) protein of the influenza virus leads to inhibition of polyadenylation event and 3′ end cleavage of pre-mRNA associated with the host. Elevated expression of CPSF4 is observed in lung adenocarcinomas.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable