S9007

Spectinomycin -dihydrochlorid Pentahydrat

potency: ≥603 μg per mg

• Synonym(e): Actinospectacin, Actinospectacin dihydrochloride pentahydrate, Spectinomycin, Spectinomycin HCL, Spectinomycin Hydrochloride, Spectinomycin hydrochloride
• Empirische Formel (Hill-System): C₁₄H₂₄N₂O₇ · 2HCl · 5H₂O
• CAS-Nummer: 22189-32-8
• Molekulargewicht: 495.35
• NACRES: NA.85
• PubChem Substance ID: 24899822
• UNSPSC Code: 51281666
• EC Number: 244-554-3
• MDL number: MFCD00150886

Eigenschaften

• biological source: synthetic (inorganic)
• Quality Segment: 200
• form: powder
• potency: ≥603 μg per mg
• storage condition: (Keep container tightly closed in a dry and well-ventilated place.)
• color: white to pale buff
• solubility: H₂O: soluble 50 mg/mL
• antibiotic activity spectrum: Gram-negative bacteria, Gram-positive bacteria
• application(s): agriculture
• mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: O.O.O.O.O.Cl.Cl.CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@]3(O)[C@@H](O[C@H](C)CC3=O)O[C@@H]2[C@H]1O
• InChI: 1S/C14H24N2O7.2ClH.5H2O/c1-5-4-6(17)14(20)13(21-5)22-12-10(19)7(15-2)9(18)8(16-3)11(12)23-14;;;;;;;/h5,7-13,15-16,18-20H,4H2,1-3H3;2*1H;5*1H2/t5-,7-,8+,9+,10+,11-,12-,13+,14+;;;;;;;/m1......./s1
• InChI key: DCHJOVNPPSBWHK-UXXUFHFZSA-N

Beschreibung

General description

Chemical structure: aminoglycoside

Application

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

Biochem/physiol Actions

Wirkungsweise: Spectinomycin wirkt durch Hemmung der Proteinsynthese und -verlängerung, indem es an die bakterielle 30S-Ribosomen-Untereinheit bindet und in die Peptidyl-tRNA-Translokation eingreift.

Resistenzmethode: Eine Mutation in rpsE, dem Gen für das ribosomale Protein S5, verhindert die Bindung von Spectinomycin. Resistenz erfolgt auch durch das Aminoglykosid-3-′-Adenyltransferase-Gen (aadA).

Antimikrobielles Spektrum: Spectinomycin wirkt gegen gramnegative und grampositive Bakterien.

Preparation Note

Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.

Disclaimer

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)