S1277

Streptomycin -sulfat (Salz)

powder, BioXtra, suitable for mouse embryo cell culture

• Lineare Formel: C₂₁H₃₉N₇O₁₂ · 1.5 H₂SO₄
• CAS-Nummer: 3810-74-0
• Molekulargewicht: 728.69
• UNSPSC Code: 12352207
• NACRES: NA.75
• PubChem Substance ID: 24899470
• EC Number: 223-286-0
• Beilstein/REAXYS Number: 3894995
• MDL number: MFCD00037023

Eigenschaften

• product line: BioXtra
• Quality Segment: 200
• form: powder
• potency: ≥720 I.U. per mg
• technique(s): cell culture | embryo: suitable
• antibiotic activity spectrum: Gram-negative bacteria, Gram-positive bacteria, mycobacteria
• mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@H]2[C@@H](O[C@@H](C)[C@]2(O)C=O)O[C@@H]3[C@@H](O)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]3NC(N)=N.CN[C@H]4[C@H](O)[C@@H](O)[C@H](CO)O[C@H]4O[C@H]5[C@@H](O[C@@H](C)[C@]5(O)C=O)O[C@@H]6[C@@H](O)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]6NC(N)=N
• InChI: 1S/2C21H39N7O12.3H2O4S/c2*1-5-21(36,4-30)16(40-17-9(26-2)13(34)10(31)6(3-29)38-17)18(37-5)39-15-8(28-20(24)25)11(32)7(27-19(22)23)12(33)14(15)35;3*1-5(2,3)4/h2*4-18,26,29,31-36H,3H2,1-2H3,(H4,22,23,27)(H4,24,25,28);3*(H2,1,2,3,4)/t2*5-,6-,7-,8+,9-,10-,11-,12+,13-,14-,15-,16-,17-,18-,21+;;;/m00.../s1
• InChI key: QTENRWWVYAAPBI-YZTFXSNBSA-N

Beschreibung

General description

Chemical structure: aminoglycoside

Application

Streptomycin is used to study mechanisms of streptomycin resistance and is often used together with penicillin and other agents to inhibit bacterial contamination in cell culture applications. It is recommended for use in molecular biology applications at 25-50μg/mL, in cell culture applications at 100 mg/L and in embryo culture at 50 mg/L.

Biochem/physiol Actions

Wirkungsweise: Streptomycin wirkt durch die Hemmung der Prokaryoten-Proteinsynthese, indem es an das S12-Protein der 30S-Untereinheit des Ribosoms bindet und dadurch die Translation vom Initiationskomplex zum kettenverlängernden Ribosom verhindert. Dadurch entsteht eine Fehlcodierung bzw. die Initiation wird gehemmt.

Widerstandsmechanismus: Eine Mutation in rpsL, einem Gen für das ribosomale Protein S12, verhindert die Bindung von Streptomycin an das Ribosom. Durch eine Aminoglycosid-Phosphotransferase wird Streptomycin ebenfalls inaktiviert.

Antimikrobielles Spektrum: Streptomycin wirkt gegen gramnegative und grampositive Bakterien.

Preparation Note

Stock solutions should be prepared with sterile water at concentrations between 20-25 mg/ml.

Disclaimer

Solutions can be stored at 2-8°C for up to a month or at -20°C for more extended periods. At 37°C, solutions are stable for three days.

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Warning
• hcodes: H302,H361fd
• pcodes: P202 - P264 - P270 - P280 - P301 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Repr. 2
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves