R5258

Riboflavin Binding Protein from chicken egg white

lyophilized powder

• Synonym(e): Binding Protein, Chicken Egg White Protein, Riboflavin Protein
• NACRES: NA.26
• UNSPSC Code: 12352202
• Form: lyophilized powder
• Biological source: chicken egg white

Eigenschaften

• biological source: chicken egg white
• form: lyophilized powder
• concentration: ≥50% protein (biuret)
• UniProt accession no.: P02752
• storage temp.: −20°C
• Gene Information: chicken ... RBP(396449)

Beschreibung

General description

Riboflavin binding protein (RBP) transports riboflavin through the bloodstream of the laying hen into the eggs, where the vitamin is essential for embryonic growth and development. RBP consists of a single polypeptide chain containing 9 disulfide bonds. It is a glycoprotein containing ~14% carbohydrate, with sugar moieties including galactose, mannose and glucosamine, and is phosphorylated.

Riboflavin Binding Protein (RBP) comprises of 19 cysteines and corresponds to a molecular weight of 30 kDa. Egg white RBP is produced in the oviduct cells. Chicken egg white RBP contains an N-terminal ligand binding domain and a C-terminal phosphorylated motif.

Application

Riboflavin Binding Protein from chicken egg white may be used in fluorescence quenching experiments.

Biochem/physiol Actions

Riboflavin Binding Protein (RBP) is crucial for normal embryo development. RBP interacts with riboflavin and its analogs. The RBP-riboflavin complex is stabilized by interaction between the isoalloxazyne ring of riboflavin and the tyrosine 75 and tryptophan 156 of residues of RBP. RBP elicits sweet supressing functionality and may interact with the sweet taste receptor.

Riboflavin binding protein binds to flavokinase and regulates its activity.

Preparation Note

Supplied as the riboflavin-depleted apo-form.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)