P7582

Amino-terminal FLAG-BAP^™ Fusion Protein

• Synonym(e): Amino-terminal Fusion Protein, BAP Fusion Protein, FLAG-BAP Fusion Protein
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00130921
• Form: liquid
• Mol wt: ~49 kDa

Eigenschaften

• form: liquid
• Quality Segment: 200
• mol wt: ~49 kDa
• shipped in: dry ice
• storage temp.: −20°C

Beschreibung

General description

Amino-terminal FLAG-BAP^™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP^™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.

The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.

Application

Amino-terminal FLAG-BAP^™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8[1] and Drosophila melanogaster transcription factor interactome.

Learn more product details in our FLAG^® application portal.

Physical form

Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.

Other Notes

Control protein

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)