P7119

PGO Enzyme Preparation

1 G capsules

• Synonym(e): Peroxidase - Glucose oxidase preparation
• NACRES: NA.54
• UNSPSC Code: 12352204

Eigenschaften

• Quality Segment: 200
• form: powder
• solubility: H₂O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)
• storage temp.: 2-8°C

Beschreibung

General description

Each capsule contains 500 units of glucose oxidase (Aspergilus niger), 100 purpurogallin units of peroxidase (horseradish), and buffer salts. The reaction produces oxidized o-Dianisidine, which is brown. The intensity of the brown color measured at 425-475 nm is proportional to the original glucose concentration.

Application

PGO Enzymes preparation has been used:

• in diet sampling and chemical analyses
• to determine plasma and follicular fluid glucose levels
• to measure glucose content in fractions of retrograded debranched rice starch

Tested and found suitable for use in the quantitation of glucose.

Biochem/physiol Actions

PGO Enzymes are used for the quantitative, enzymatic determination of glucose in aqueous solutions such as serum. The reactions are normally monitored at 425-475 nm utilizing o-dianisidine as a colorimetric substrate. Glucose oxidase methods eliminate the effects of interfering substances. Contents of one capsule includes 100 units peroxidase and 500 units glucose oxidase and buffer salts.

Preparation Note

The PGO enzymes solution is prepared by adding the contents of 1 capsule of PGO enzymes to 100 mL of water in an amber bottle. Bottle should be inverted several times with gentle shaking to dissolve. PGO Enzymes solution should be stored at 2-8 °C. The solution is stable for up to 1 month unless turbidity develops, and for at least 6 months at –20 °C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves