P4252

Phosphatase, alkalisch aus E. coli

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

• Synonym(e): Orthophosphor-Monoesterphosphohydrolase (alkalisches Optimum)
• CAS-Nummer: 9001-78-9
• UNSPSC Code: 12352204
• eCl@ss: 42010105
• EC Number: 232-631-4
• NACRES: NA.54
• MDL number: MFCD00131849
• EG-Nummer: 3.1.3.1 (BRENDA, IUBMB)
• Specific activity: 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
• Biological source: Escherichia coli

Eigenschaften

• biological source: Escherichia coli
• Quality Segment: 300
• form: ammonium sulfate suspension
• specific activity: 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
• storage temp.: 2-8°C

Beschreibung

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA . Product P4252 is provided in the ratio of 30-90 units/mg of protein.

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer.

Biochem/physiol Actions

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium[1][2]. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Physical form

Suspension in 2.5 M (NH₄)₂SO₄

Analysis Note

Die Aktivität bei pH 8.0 und 25 °C ist ungefähr die gleiche wie bei pH 10.4 und 37 °C.

Other Notes

Eine Einheit hydrolysiert 1,0 μmol p-Nitrophenylphosphat je Min. bei einem pH-Wert von 10,4 und 37 °C.

Disclaimer

Die Anweisenheit von 0.1% SDS beim Alkalischen Phosphate-Assay inhibiert jegliche Nuklease-Kontamination in der Probe.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)