This product has not been tested in serum-free, feeder-free induced pluripotent stem cell culture conditions or on stem cells/induced pluripotent stem cells. The effect on pluripotency is currently unknown. No data are available regarding compatibility with these conditions.
Fortfahren mit
MP0030
LookOut® Mycoplasma-Eliminierungskit
Synonym(e):
Mycoplasma contamination treatment
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Warenkorb auf Verfügbarkeit prüfen | € 304,00 |
Über diesen Artikel
NACRES:
NA.75
UNSPSC Code:
12352207
€ 304,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenusage
kit sufficient for 5 mL mycoplasma elimination
Quality Segment
packaging
pkg of 1 kit
storage condition
protect from light
technique(s)
PCR: suitable
application(s)
detection
microbiology
storage temp.
2-8°C
General description
Das Kit enthält eine Kombination biologischer Wirkstoffe, die Kontaminationen mit Mykoplasmen zuverlässig und vollständig entfernen. Bei den meisten Anwendungen reicht die anfängliche Behandlung mit diesem Tilgungsverfahren zur Eliminierung von Mykoplasmen. Im zweiten Schritt werden eventuell verbleibende Mykoplasmen mit einer antibiotischen Folgebehandlung unterdrückt und inaktiviert.
Application
Das LookOut® Mykoplasmen-Eliminierungskit eignet sich für die Eliminierung von Mollicutes und verwandten Organismen (Mycoplasma, Acholeplasma, Spiroplasma und Entomoplasma) in Zell- und Viruskulturen. Verunreinigungen mit Mykoplasmen in Chlamydia-Kulturen werden damit nicht effektiv behandelt.
Das LookOut® Mykoplasmen-Eliminierungskit wurde entwickelt, um Verunreinigungen mit Mykoplasmen rasch und effizient aus Zellkulturen zu entfernen.
Features and Benefits
- Minimal zytotoxische Eigenschaften der Reagenzien
- Hocheffektiv
- Beibehaltung der normalen Zelleigenschaften
Legal Information
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Das Produkt ist nicht für die Verwendung als Biozid gemäß den globalen Biozid-Vorschriften vorgesehen, u. a. dem Federal Insecticide Fungicide and Rodenticide Act der US-amerikanischen EPA, der Europäischen Biozid-Produktverordnung, der kanadischen Behörde für Schädlingsbekämpfung, der türkischen Biozid-Produktverordnung, Koreas Consumer Chemical Products and Biocide Safety Management Act (K-BPR) und andere.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| technique(s) PCR: suitable | technique(s) PCR: suitable | technique(s) DNA purification: suitable | technique(s) Rapid Sterility Testing: suitable |
| application(s) detection | application(s) detection | application(s) microbiology | application(s) bioburden testing |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - | packaging pkg of 1 kit |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. room temp | storage temp. room temp |
| usage kit sufficient for 5 mL mycoplasma elimination | usage sufficient for 25 reactions | usage - | usage - |
Lagerklasse
10 - Combustible liquids
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Is this elimination reagent compatible with serum-free, feeder-free iPSC culture conditions?
1 answer-
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After I dilute my epithelial cells in the media + primary antibiotic, do I not change the media until they reach 80-90% confluency? I usually change the media on the cells every 2-3 days.
1 answer-
The product information sheet protocol for this product instructs to grow the cells in the initial treatment media until 80–90% confluency before passaging. If a media change is necessary for cell health, it is important to include the elimination reagent in the new media.
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Has this been tested in primary cell lines (i.e. skeletal muscle) is there any interference with the normal cell physiology?
1 answer-
The LookOut Mycoplasma Elimination Kit is designed to efficiently eliminate mycoplasma contamination from cell cultures. This kit uses a combination of biological agents to reliably and completely remove mycoplasma contamination. The initial treatment is generally sufficient for mycoplasma elimination in most applications. A second step involves a follow-up antibiotic treatment to suppress and inactivate any remaining mycoplasma. The reagents have minimal cytotoxic properties, and the development of resistant strains is highly unlikely due to the significantly reduced mycoplasma concentration after the initial treatment. Compared to other products for mycoplasma elimination, inactivation, or suppression, the LookOut Mycoplasma Elimination Kit is more effective and has not been shown to cause changes in normal cell characteristics. It is the least cytotoxic method, with overall cytotoxicity demonstrated to be less than 20% across a diverse group of cell lines
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What antibiotics are included in MP0030, the LookOut® Mycoplasma Elimination Kit? Are fluoroquinolones, macrolides, or tetracyclines part of the kit?
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The MP0030 product contains two mycoplasma-active antibiotics, with one of them being a fluoroquinolone.
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How does the mechanism of action (MOA) of Product No. MP0030, LookOut® Mycoplasma Elimination Kit, differ from that of BM cyclin?
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The key distinction lies in Product No. MP0030, LookOut® Mycoplasma Elimination Kit, which actively eradicates mycoplasma by disrupting the membrane. As a lipopeptide, it identifies cholesterol-rich regions in the membrane (as the concentration of cholesterol in mycoplasma membranes is higher than in eukaryotic cells), creating a pore through which the surrounding medium flows into the mycoplasma, ultimately causing it to burst. In contrast, standard antibiotics simply inhibit metabolic pathways, necessitating the rinsing out of mycoplasma through a medium change. If the process is halted prematurely, any remaining mycoplasma can regrow.
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