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M9288

Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

Synonym(e):

MEM

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Über diesen Artikel

UNSPSC Code:

12352207

NACRES:

NA.75

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Eigenschaften

Produktname

Minimum Essential Medium Eagle, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO₃: no
Hanks’ salts (2% CO₂): yes
L-glutamine: no
HEPES: no
sodium pyruvate: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Beschreibung

General description

Minimum Essential Medium (MEM), one of the most used synthetic cell culture media, is developed by Harry Eagle. It is used to cultivate various types of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used as a transport media to maintain pig liver cells.[1]

Preparation Note

Supplement with 0.292 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.

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Dieser Artikel	M4655	M2414	M0325
Sigma-Aldrich M9288 Minimum Essential Medium Eagle	Sigma-Aldrich M4655 Minimum Essential Medium Eagle	Sigma-Aldrich M2414 Minimum Essential Medium Eagle	Sigma-Aldrich M0325 Minimum Essential Medium Eagle
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
form liquid	form liquid	form liquid	form liquid
components NaHCO₃: no Hanks’ salts (2% CO₂): yes L-glutamine: no HEPES: no sodium pyruvate: no phenol red: yes	components sodium pyruvate: no phenol red: yes Earle’s salts (5% CO₂): yes HEPES: no NaHCO₃: yes L-glutamine: yes	components phenol red: 0.011 g/L NaHCO₃: 0.85 g/L glucose: 1.0 g/L L-glutamine: no	components glucose: 1.0 g/L (Dextro) L-glutamine: 0.292 g/L phenol red: 0.011 g/L Earle’s salts (5% CO₂): yes NaHCO₃: 2.2 g/L
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

Anonymous

2 years ago

If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?

1 answer
1. Technical Support
  
  2 years ago
  
  Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
  Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
  (NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
  • Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
  • While gently stirring the water, add 100 ml of 10X medium.
  • To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
  • While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
  • Bring medium to final volume with additional tissue culture grade water.
  • It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
  • Aseptically dispense into sterile containers. Store refrigerated at 2-8° C
  
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