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M8537

Sigma-Aldrich

MCDB 131 Medium

With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

product name

MCDB 131 Medium, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Form

powder

Konzentration

11.6 g/L

Methode(n)

cell culture | mammalian: suitable

Komponenten

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

Versandbedingung

ambient

Lagertemp.

2-8°C

Allgemeine Beschreibung

MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). 

Anwendung

MCDB-131 Medium has been used:

  • as a component of the basal medium to culture bovine retinal endothelial cells
  • to culture bovine lung microvascular endothelial cells
  • for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells

Menge

Formulated to contain 11.6 grams of powder per liter of medium.

Rekonstituierung

Supplement with 1.18 g/L sodium bicarbonate.

Ergänzung

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

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Kathleen M Job et al.
Anesthesia and analgesia, 123(2), 382-393 (2016-06-23)
The endothelial glycocalyx is an important component of the vascular permeability barrier, forming a scaffold that allows serum proteins to create a gel-like layer on the endothelial surface and transmitting mechanosensing and mechanotransduction information that influences permeability. During acute inflammation
Thomas P Burke et al.
eLife, 10 (2021-08-24)
Arthropod-borne rickettsial pathogens cause mild and severe human disease worldwide. The tick-borne pathogen Rickettsia parkeri elicits skin lesions (eschars) and disseminated disease in humans; however, inbred mice are generally resistant to infection. We report that intradermal infection of mice lacking
Sandra S Hammer et al.
Diabetologia, 64(7), 1674-1689 (2021-03-27)
Homo sapiens evolved under conditions of intermittent food availability and prolonged fasting between meals. Periods of fasting are important for recovery from meal-induced oxidative and metabolic stress, and tissue repair. Constant high energy-density food availability in present-day society contributes to
G S Ajithkumar et al.
Cardiovascular toxicology, 16(4), 390-405 (2015-12-15)
Endocardial endothelial cells (EECs), when compared with endothelial cells of arteries and veins, possess higher resistance to apoptosis-inducing anticancer agents. The mechanism of this resistance property is unknown. We have investigated the molecular mechanism, which contributes to increased cell survival
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Protokolle

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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