M7279

N,N′-Methylenbisacrylamid

powder, Molecular Biology, suitable for electrophoresis, ≥99.5%

• Synonym(e): BIS
• Lineare Formel: (CH₂=CHCONH)₂CH₂
• CAS-Nummer: 110-26-9
• Molekulargewicht: 154.17
• UNSPSC Code: 41105319
• NACRES: NB.22
• PubChem Substance ID: 24897151
• EC Number: 203-750-9
• Beilstein/REAXYS Number: 1706297
• MDL number: MFCD00008625

Eigenschaften

• grade: Molecular Biology
• Quality Segment: 200
• assay: ≥99.5%
• form: powder
• technique(s): electrophoresis: suitable
• impurities: ≤0.05% Free carboxylic acid (T), ≤50 mg/kg Total sulfur (as SO₄ ) (ICP)
• conductivity: ≤10 μmho (2% solution)
• anion traces: chloride (Cl^-): ≤50 mg/kg
• cation traces: Ca: ≤10 ppm, Cd: ≤5 ppm, Co: ≤5 ppm, Cr: ≤5 ppm, Cu: ≤5 ppm, Fe: ≤5 ppm, K: ≤50 ppm, Mg: ≤5 ppm, Mn: ≤5 ppm, Na: ≤50 ppm, Ni: ≤5 ppm, Pb: ≤5 ppm, Zn: ≤5 ppm
• absorption: ≤0.2 at 290 at 1%
• foreign activity: DNase and RNase, none detected
• SMILES string: C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)
• InChI key: ZIUHHBKFKCYYJD-UHFFFAOYSA-N

Beschreibung

General description

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.

Application

Zum Mischen mit Acrylamid für die Herstellung von Polyacrylamidgelen für den Einsatz in Protein- und Nukleinsäure-Elektrophorese.

N,N′-Methylenebisacrylamide has been used:

• as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix[1]
• as a crosslinker to prepare tough hydrogel network
• in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel

Sicherheitshinweise

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H301,H312,H340,H350,H361fd,H372
• pcodes: P202 - P260 - P264 - P280 - P301 + P310 - P302 + P352 + P312
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Carc. 1B - Muta. 1B - Repr. 2 - STOT RE 1 Oral
• target_organs: Peripheral nervous system
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves