M7133

4-Methylumbelliferylsulfat Kaliumsalz

sulfatase substrate

• Synonym(e): Potassium 4-methylumbelliferyl sulfate
• Empirische Formel (Hill-System): C₁₀H₇KO₆S
• CAS-Nummer: 15220-11-8
• Molekulargewicht: 294.32
• Beilstein: 3803203
• EG-Nummer: 239-272-2
• MDL-Nummer: MFCD00016970
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 24897141
• NACRES: NA.32

Eigenschaften

• Assay: ≥99% (HPLC)
• Form: powder
• Löslichkeit: water: 5 mg/mL, clear, colorless
• Fluoreszenz: λ_ex 334 nm; λ_em 370 nm (pH 10.4); λ_ex 360 nm; λ_em 449 nm (Reaction products)
• Lagertemp.: −20°C
• SMILES String: [K+].CC1=CC(=O)Oc2cc(OS([O-])(=O)=O)ccc12
• InChI: 1S/C10H8O6S.K/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14;/h2-5H,1H3,(H,12,13,14);/q;+1/p-1
• InChIKey: CSOCSPXOODWGLJ-UHFFFAOYSA-M

Beschreibung

Allgemeine Beschreibung

4-Methylumbelliferyl sulfate (4MUS), a general substrate, is a polar metabolite of 4-methylumbelliferone (4MU).

Anwendung

4-Methylumbelliferyl sulfate potassium salt has been used as a substrate in coupled fluorescence sulfotransferase assay to regenerate 3′-phosphoadenosine 5′-phosphosulfate (PAPS) from 3′-phosphoadenosine 5′-phosphate for cerebroside sulfotransferase (CST) enzyme activity measurements. It has also been used as a substrate in arylsulfatase assay/4-methylumbelliferyl sulfate (4-MUS) assay to test the enzymatic activity of the protein-bead complex.

Biochem./physiol. Wirkung

4-Methylumbelliferyl sulfate (4MUS) is capable of undergoing desulfation and involve in futile cycling with 4MU. It can be utilized to measure the arylsulfatase activity of several sulfatases.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)