M6133

7-Methylguanosine 5′-triphosphate sodium salt

≥85% (HPLC), natural (inorganic), powder

• Synonym(e): m7GTP
• Empirische Formel (Hill-System): C₁₁H₁₈N₅O₁₄P₃ · xNa⁺
• CAS-Nummer: 104809-18-9
• Molekulargewicht: 537.21 (free acid basis)
• UNSPSC Code: 41106305
• PubChem Substance ID: 24897079
• NACRES: NA.51
• MDL number: MFCD00063376
• Assay: ≥85% (HPLC)
• Biological source: natural (inorganic)
• Form: powder
• Solubility: water: 50 mg/mL, clear, colorless to faint yellow or tan
• Storage temp.: −20°C

Eigenschaften

• Produktname: 7-Methylguanosine 5′-triphosphate sodium salt, ≥85% (HPLC)
• biological source: natural (inorganic)
• assay: ≥85% (HPLC)
• form: powder
• solubility: water: 50 mg/mL, clear, colorless to faint yellow or tan
• storage temp.: −20°C
• SMILES string: [Na+].C[n+]1cn([C@@H]2O[C@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)[C@@H](O)[C@H]2O)c3N=C(N)NC([O-])c13
• InChI: 1S/C11H20N5O14P3.Na/c1-15-3-16(8-5(15)9(19)14-11(12)13-8)10-7(18)6(17)4(28-10)2-27-32(23,24)30-33(25,26)29-31(20,21)22;/h3-4,6-7,9-10,17-18H,2H2,1H3,(H,23,24)(H,25,26)(H3,12,13,14)(H2,20,21,22);/q;+1/p-1/t4-,6-,7-,9?,10-;/m1./s1
• InChI key: FSMRTZAMTMQMEF-OCMBMSOASA-M

Beschreibung

General description

7-Methylguanosine 5′-triphosphate is a mRNA cap structural analog.

Application

7-Methylguanosine 5′-triphosphate sodium salt has been used to test its affinity towards Poly(A)-specific ribonuclease (PARN) polypeptides using fluorescence titrations. It has also been used in crystallization along with gemin5 fragment (G5N) and DM15 region of human La-related 15 protein 1 ( LARP1).

7-Methylguanosine 5′-triphosphate (m7GTP) is use to study the structures and mechanisms of eukaryotic cellular mRNA caps in the context of mRNA involvement in protein synthesis. m7GTP may be used to create affinity media for capture and purification of molecules such as eukaryotic initiation factor eIF4E.

Biochem/physiol Actions

7-Methylguanosine 5′-triphosphate (m7GTP) inhibits in vitro protein synthesis by binding to translation initiation complexes. m7GTP is useful to study the structures and mechanisms of eukaryotic cellular mRNA caps in the context of mRNA involvement in protein synthesis. m7GTP has a high affinity for eukaryotic initiation factor eIF4E and may be used to create affinity media for their capture and purification.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• Hazard Classifications: Acute Tox. 4 Oral
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves
• pcodes: P264 - P270 - P301 + P312 - P501