M4943

Anti-phospho-MEK4 (pSer²⁵⁷/pThr²⁶¹) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.44
• MDL number: MFCD06798497
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 3

Eigenschaften

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human, frog, mouse
• technique(s): western blot: 0.1-1.0 μg/mL using extracts prepared from GST-tagged fusion protein expressing MEK 4.
• UniProt accession no.: P45985
• shipped in: dry ice
• storage temp.: −70°C
• Quality Level: 200
• Gene Information: human ... MAP2K4(6416) ; mouse ... Map2k4(26398)

Beschreibung

General description

Mitogen-Activated Protein Kinase Kinase 4 (MEK 4 or MKK4) is a 42 kDa member of a tyrosine/threonine protein kinase family that activates the c-Jun NH2-terminal kinases (JNK), which is part of the inflammation/stress signaling pathway. The mutation of the MEK 4 gene in some carcinomas indicates that it may also have a role as a tumor suppressor. Phosphorylation of MEK4 by MEKK1 on serine 257 and threonine 261 in the catalytic domain activates the protein and enables MEK 4 to phosphorylate JNK
This antibody specifically recognizes human MEK 4 double phosphorylated at serine 257 and threonine 261. Mouse and frog MEK 4 (100% homology) may cross react.

Immunogen

synthetic MEK4-derived phosphopeptide that is phosphorylated at serine 257 and threonine 261.

Application

Anti-phospho-MEK4 (Tyr³⁹⁷) antibody may be used for detection by immunoblotting at a working concentration of 0.1-1.0 μg/mL.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids