M1255

Calceinblau

Powder

• Synonym(e): 7-Hydroxy-4-methyl-8-cumarinylmethyliminodiessigsäure
• Empirische Formel (Hill-System): C₁₅H₁₅NO₇
• CAS-Nummer: 54375-47-2
• Molekulargewicht: 321.28
• EG-Nummer: 259-129-8
• MDL-Nummer: MFCD00006867
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24896629
• NACRES: NA.47

Eigenschaften

• product name: Calceinblau, fluorescent dye
• Form: powder
• Farbe: white to light yellow
• Löslichkeit: 1 M NaOH: 50 mg/mL
• Fluoreszenz: λ_ex 322 nm; λ_em 445 nm in methanol
• Eignung: suitable for indicator for EDTA titration
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: room temp
• SMILES String: CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
• InChI: 1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
• InChIKey: RJPSHDMGSVVHFA-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.

Anwendung

Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H315,H319,H335
• P-Sätze: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves