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L6007

Leucin-Aminopeptidase, mikrosomal aus Schweineniere

Name: Leucin-Aminopeptidase, mikrosomal aus Schweineniere
Brand: SIGMA
Price: 509 EUR

Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

Synonym(e):

(R)-2-Amino-3-(4-methylphenylthio)-propionsäure, Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinaminopeptidase

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Ihnen/SKU	Verfügbarkeit	Preis
25 units	Warenkorb auf Verfügbarkeit prüfen	€ 509,00
50 units	Warenkorb auf Verfügbarkeit prüfen	€ 760,00
100 units	Warenkorb auf Verfügbarkeit prüfen	€ 1.400,00

Über diesen Artikel

CAS-Nummer:

9054-63-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-942-5

MDL number:

MFCD00131501

EG-Nummer:

3.4.11.2 (BRENDA, IUBMB)

Specific activity:

≥12 units/mg protein (biuret)

Biological source:

Porcine kidney

€ 509,00

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Eigenschaften

biological source

Porcine kidney

Quality Segment

200

type

Type VI-S

form

lyophilized powder

specific activity

≥12 units/mg protein (biuret)

mol wt

320 kDa

composition

Protein, 30-70%

storage temp.

−20°C

Beschreibung

General description

Leucine Aminopeptidase (LAP) from porcine is a 320-kDa exopeptidase present in cytosol. It contains zinc in its structure and belongs to the M1 and M17 peptidase families. LAP display bi-lobed structure and has the catalytic domain at the C-terminus and a variable N-terminal domain.

Application

Leucine Aminopeptidase, microsomal from porcine kidney has been used in aminopeptidase N (APN) inhibitor screening assay in adenocarcinoma cell line ES-2 and in the inhibition kinetic studies with APN inhibitor.[1]

Biochem/physiol Actions

Leucine Aminopeptidase (LAP) catalyzes protein hydrolysis at the leucine residue at N-terminal. An altered level of LAP is implicated in tumor proliferation and progression. LAP is a potential biomarker for ovarian epithelial cancer therapy. Porcine LAP hydrolyzes glycine and arginine substrates.

Physical form

Contains primarily phosphate buffer salts, pH 7.0

Other Notes

Eine Einheit hydrolysiert 1.0 μmol L-Leucin-p-nitroanilid pro Min. zu L-Leucin und p-Nitroanilin bei pH 7.2 und 37 °C. (Bei 25°C, wird ca. 40% der bei 37 °C gefundenen Aktivität erreicht.) Die Aktivität bei Verwendung von L-Leucin-p-nitroanilid als Substrat ist 2-5 Mal höher als bei der Verwendung von L-Leucinamid als Substrat.

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Dieser Artikel	P6675	L5006	164598
Sigma-Aldrich L6007 Leucin-Aminopeptidase, mikrosomal aus Schweineniere	Sigma-Aldrich P6675 Prolidase aus Schweineniere	Sigma-Aldrich L5006 Leucin-Aminopeptidase, mikrosomal aus Schweineniere	Sigma-Aldrich 164598 Aminopeptidase M, Porcine Kidney
biological source Porcine kidney	biological source -	biological source -	biological source Porcine kidney
specific activity ≥12 units/mg protein (biuret)	specific activity ≥100 units/mg protein	specific activity 10-40 units/mg protein (Bradford)	specific activity -
form lyophilized powder	form lyophilized powder	form ammonium sulfate suspension	form suspension (crystalline)
mol wt 320 kDa	mol wt -	mol wt -	mol wt -
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions

Anonymous

a year ago

I would like to know the optimum solvent for dissolving, storing and using this reagent (Cat # L6007-25UN).

1 answer
1. Technical Support
  
  a year ago
  
  According to the Enzymatic Assay for Leucine Aminopeptidase, Microsomal Enzyme Solution, prepare a solution containing 0.10 - 0.15 unit/ml of Leucine Aminopeptidase, Microsomal in cold deionized water immediately before use.
  More information can be found at https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/356/218/l6007enz.pdf.
  This formulation contains phosphate buffer salts. For reconstitution, storage, and use of this product, 50 mM Sodium Phosphate Buffer, pH7.2, is a suitable solvent.
  
  Helpful?
3 years ago

I recently purchased Leucine Aminopeptidase from porcine kidney Cat # L6007-25UN. I'd like to inquire how many mgs of protein is in one vial.

1 answer
1. Technical Support
  
  3 years ago
  
  The milligrams of protein per vial will depend on the units/mg protein defined on the product's certificate of analysis. For example, lot SLCM3898 reports specific activity of 19 units/mg protein. This results in approximately 1.3 mg of protein per vial for this particular lot. The protein content of this lot of material is reported at 61%, therefore the total mass of solid material in the 25UN vial will total around 2.2 mg.
  (Link to certificate of analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/L6007-BULKSLCM3898.pdf)
  
  Helpful?

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