H5666

Granulozyten-Makrophagen-Kolonie-stimulierender Faktor human

HumanKine^®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonym(e): GM-CSF
• UNSPSC Code: 12352202
• NACRES: NA.77
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: dimer 15-36 kDa (glycosylated)
• Impurities: ≤1 EU/mg

Eigenschaften

• Produktname: Granulozyten-Makrophagen-Kolonie-stimulierender Faktor human, GM-CSF, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• biological source: human
• Quality Segment: 100
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤5 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 15-36 kDa (glycosylated)
• packaging: pkg of 5X10 μg, pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/mg
• UniProt accession no.: P04141
• storage temp.: −20°C
• Gene Information: human ... CSF2(1437)

Beschreibung

General description

Granulocyte-Macrophage Colony-Stimulating Factor is mapped to human chromosome 5q21-q32.

Application

Granulocyte-Macrophage Colony-Stimulating Factor human has been used as a colony-stimulating cytokine in human acute myeloid leukemia cell line (OCI-M2) to test its effect on 5-azacitidin (AZA)-mediated DNA demethylation of upstream regulatory element (URE).

Biochem/physiol Actions

Der Granulozyten-Makrophagen-Kolonie-stimulierende Faktor (GM-CSF) ist ein Wachstums- und Differenzierungsfaktor für Zellen der Granulozyten-, Makrophagen- und Eosinophilen-Linie. GM-CSF stimuliert bei extrem niedrigen Konzentrationen die Koloniebildung aus pluripotenten Vorläuferzellen und ist ein essenzieller Faktor für Überleben und Proliferation von hämatopoietischen Vorläuferzellen in allen Stadien bis hin zur Reife. Ebenso stimuliert er das Wachstum mancher Epithelzellen und Osteoplasten. GM-CSF wird von Zellen verschiedener Typen produziert (Monozyten, Endothelzellen, T-Zellen, Fibroblasten, Mitogen-stimulierte B-Zellen und LPS-stimulierte Makrophagen). GM-CSF wird als einkettiges Glycoprotein mit 128 Aminosäuren (beim Menschen) und einer konservierten Disulfidbindung sezerniert. GM-CSF von Mensch und Maus weisen eine Sequenzhomologie von ca. 54 % auf, eine Kreuzreaktivität bei Bioaktivität liegt nicht vor.

Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is an immune modulator and proinflammatory cytokine. It binds to the heteromeric cell-surface receptors present in the immune cells like granulocytes, endothelial cells and macrophages. GM-CSF is implicated in inflammation associated disorder of the lung and renal system.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine GM-CSF is expressed as a 15-36 kDa glycosylated monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Disclaimer

Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable