G8296

GRK5, active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): GPRK5
• NACRES: NA.32
• UNSPSC Code: 12352200
• Specific activity: 32-44 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

Eigenschaften

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 32-44 nmol/min·mg
• mol wt: ~95 kDa
• UniProt accession no.: P34947
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... GRK5(2869)

Beschreibung

General description

GRK5 (G protein-coupled receptor kinase 5) belongs to the G-protein coupled receptor kinase family, and shows major expression in normal human heart. This protein contains a nuclear localization sequence and hence localizes in nucleus.

Biochem/physiol Actions

GRK5 (G protein-coupled receptor kinase 5) controls the functions of catecholamines, which bind and activate β-adrenergic receptors, and have cardiac inotropic and chronotropic actions. It is responsible for the uncoupling from G protein by facilitating β1-adrenergic receptor phosphorylation and β-arrestin recruitment. This protein plays a key role in growth and metastasis in prostate cancer, where it phosphorylates moesin.† A genome-wide association study (GWAS) has shown that rs10886471 variant in GRK5 gene is linked with susceptibility to type 2 diabetes mellitus (T2DM).

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)