G8162

ββ-Glucuronidase aus E. coli

aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret)

• Synonym(e): β-D-Glucuronide glucuronosohydrolase
• CAS-Nummer: 9001-45-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.2.1.31 (BRENDA, IUBMB)
• MDL number: MFCD00199013
• Specific activity: ≥5,000,000 units/g protein, pH 6.8 (biuret)
• Biological source: Escherichia coli

Eigenschaften

• biological source: Escherichia coli
• Quality Segment: 200
• form: aqueous glycerol solution
• specific activity: ≥5,000,000 units/g protein, pH 6.8 (biuret)
• mol wt: 69-71 kDa
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

General description

β-Glucuronidase from Escherichia coli is similar to human glucuronidase enzyme and corresponds to molecular weight close to 69-71 kDa and has an pH optimum of 6.5-7.5. It belongs to family-2 glycosyl hydrolase and has active site residues glutamic acid 394, tyrosine 468 and glutamic acid 504.

Application

β-Glucuronidase from Escherichia coli has been used in the enzymatic cleavage and activation of glucuronide prodrugs in non-small cell lung cancer cells, U87 human glioblastoma cell line, in A549 (human lung adenocarcinoma) and KB (human oral squamous carcinoma).

β-Glucuronidase from E. coli is used for the enzymatic hydrolysis of b-glucuronides in urine and other fluids. It has a high rate of hydrolytic activity and may be useful for determining the presence of androsterone, 17-hydroxycorticosteroids, and estriol in urine.
The optimal conditions for the enzymatic hydrolysis of α-hydroxytriazolam, one of the major metabolites of triazolam in human urine, were determined using β-glucuronidase Type IX-A.
It is used as a reporter gene in GUS assays to monitor gene expression.

Neuer technischer Artikel mit einem Vergleich der Leistung verschiedener Enzyme
Weitere Informationen
über neue von Sigma R&D generierte Anwendungsdaten zur Optimierung der Hydrolyse für verschiedene Arzneimittelklassen, bei denen Enzyme aus unterschiedlichen Quellen verwendet werden, und die Verwendung eines chromatographisch gereinigten Enzyms zur Verminderung der Wirkung der Esterase-Aktivität, die zur Umwandlung von 6-MAM in Morphin führt

Wird zur Hydrolyse von Glucuronidkonjugaten bei der Analyse von Harnmetaboliten verwendet

Wirksam bei der Hydrolyse von Steroid-Glucuronide

Biochem/physiol Actions

β-Glucuronidase catalyzes the hydrolysis of β-glucuronic acid residues from the non-reducing termini of glycosaminoglycans (GAGs). β-Glucuronidase is a potential candidate enzyme for gene-mediated enzyme prodrug therapy for glucuronide prodrugs. Use of β-Glucuronidase-albumin complex based drug delivery could be an effective therapeutic methodology for treating solid tumors.

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Physical form

Hochgereinigte Lösung in 50% Glycerin.

Other Notes

1 Sigma- oder modifizierte Fishman-Einheit setzt 1.0 μg Phenolphthalein aus Phenolphthalein-glucuronid pro Stunde bei 37°C und pH 6.8 (30 Min. Assay) frei.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable