G5537

Glyceraldehyde-3-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

lyophilized powder, 70-140 units/mg protein

• Synonym(e): D-Glyceraldehyde 3-phosphate:NAD⁺ oxidoreductase (phosphorylating), GAPDH
• CAS-Nummer: 9001-50-7
• UNSPSC Code: 12352204
• EC Number: 232-609-4
• MDL number: MFCD00131203
• EG-Nummer: 1.2.1.12 (BRENDA, IUBMB)
• Specific activity: 70-140 units/mg protein

Eigenschaften

• form: lyophilized powder
• Quality Segment: 200
• specific activity: 70-140 units/mg protein
• composition: Protein, 30-70% biuret
• foreign activity: 3-Phosphoglyceric phosphokinase ≤0.05%
• storage temp.: −20°C

Beschreibung

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<,New>>>.

Physical form

Lyophilized, sulfate-free powder stabilized with trehalose, citrate, and DTT. Useful for systems requiring low sulfate.

Other Notes

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Sicherheitshinweise

• Lagerklasse: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)