This is a Novozyme product, Sweetzyme® IT Extra, which is an immobilized enzyme. It is glutaraldehyde crosslinked on polyethyleneimine, and intended to be used in a continuous fixed bed process (converting high dextrose syrup to high fructose syrup).
G4166
Glucose Isomerase from Streptomyces murinus
≥350 U/g
Synonym(e):
Sweetzyme® IT Extra, D-xylose ketol-isomerase
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Specific activity:
≥350 U/g
Biological source:
Streptomyces sp. (S. murinus)
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Unterstützung erhaltenbiological source
Streptomyces sp. (S. murinus)
Quality Level
form
powder
specific activity
≥350 U/g
storage temp.
2-8°C
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥350 U/g | specific activity ≥400 units/mg protein (biuret) | specific activity ≥200 units/mg protein | specific activity ~10,000 units/mg protein |
| biological source Streptomyces sp. (S. murinus) | biological source bakers yeast | biological source rabbit muscle | biological source - |
| form powder | form ammonium sulfate suspension | form lyophilized powder | form ammonium sulfate suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
0.33 g yields an approximate bed volume of 1ml
Immobilized glucose isomerase produced from a selected strain of Streptomyces murinus.
Application
Immobilized glucose isomerase produced from Streptomyces murinus was used for the isomerization of xylose.[1] Glucose isomerase is used in the food industry to produce high-fructose corn syrup[2].
Glucose Isomerase from Streptomyces murinus has been used to isomerize xylose to xylulose during the production of dihydrogen from Xylose. It has also been used in the synthetic enzymatic pathway for dihydrogen production from sucrose, to catalyze isomerization of glucose to fructose.
Biochem/physiol Actions
Few anaerobic bacteria, fungi and plants express an intracellular metalloenzyme called D-xylose isomerase (XI). Most bacteria use the enzyme D-xylose isomerase to transform D-xylose to D-xylulose. D-Xylose isomerase (XI) converts the aldo-sugars xylose and glucose to their keto analogs xylulose and fructose.[3]
Glucose isomerase has wide variety of industrial applications such as producing high-fructose corn syrup (HFCS) and production of ethanol from hemicelluloses. In addition, it also facilitates the study of structure-function relationships by advanced biochemical and genetic engineering techniques.
Other Notes
one unit converts glucose to fructose at an initial rate of 1 μmole per min at standard analytical conditions
Legal Information
A product of Novozyme Corp.
Sweetzyme is a registered trademark of Novozymes Corp.
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Leonardo de Figueiredo Vilela et al.
Bioresource technology, 128, 792-796 (2012-11-29)
This study presents results regarding the successful cloning of the bacterial xylose isomerase gene (xylA) of Burkholderia cenocepacia and its functional expression in Saccharomyces cerevisiae. The recombinant yeast showed to be competent to efficiently produce ethanol from both glucose and
Kripa Rao et al.
Applied biochemistry and biotechnology, 146(1-3), 101-117 (2008-04-19)
Of the sugars recovered from lignocellulose, D-glucose can be readily converted into ethanol by baker's or brewer's yeast (Saccharomyces cerevisiae). However, xylose that is obtained by the hydrolysis of the hemicellulosic portion is not fermentable by the same species of
Molecular and industrial aspects of glucose isomerase.
Bhosale SH,et al.
Microbiology and Molecular Biology Reviews, 60(2), 280-300 (1996)
Dominik Oberthuer et al.
PloS one, 7(6), e33545-e33545 (2012-06-08)
In this paper, we demonstrate the feasibility of using in situ Dynamic Light Scattering (DLS) to monitor counter-diffusion crystallization experiments in capillaries. Firstly, we have validated the quality of the DLS signal in thin capillaries, which is comparable to that
Hiroki Kitayama et al.
Biochimica et biophysica acta, 1834(12), 2640-2646 (2013-10-08)
Protein crystals form in supersaturated solutions via a nucleation and growth mechanism. The amyloid fibrils of denatured proteins also form via a nucleation and growth mechanism. This similarity suggests that, although protein crystals and amyloid fibrils are distinct in their
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G4166-50G | 04061833630587 |
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Is G4166 an immobilized enzyme? If it is, what support is it immobilized on?
1 answer-
Helpful?
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グルコースイソメラーゼ Streptomyces murinus由来(Cat.No G4166) が可溶化できません。どのように溶かすのでしょうか？またどのような溶液だと可能でしょうか？
1 answer-
This is a product of Novozymes, available commercially as Sweetzyme. This is an immobilized enzyme used in a fixed bed process to convert dextrose syrup to fructose syrup. This material is in granular form with a particle size range of 0.3-1.0 mm. This material is not intended to be solubilized.
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