G4013

Glutathione Peroxidase from human erythrocytes

lyophilized powder, ≥30 units/mg protein

• Synonym(e): GSH-Px, Glutathione:hydrogen-peroxide oxido-reductase
• CAS-Nummer: 9013-66-5
• UNSPSC Code: 12352204
• MDL number: MFCD00131195
• EG-Nummer: 1.11.1.9 (BRENDA, IUBMB)

Eigenschaften

• form: lyophilized powder
• specific activity: ≥30 units/mg protein
• composition: Protein, 13-25% modified Warburg-Christian
• UniProt accession no.: P07203
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... GPX1(2876)

Beschreibung

General description

GPX1 (glutathione peroxidase 1) belongs to the four membered family of GPXs, including GPX2, GPX3 and GPX4. It is a selenocysteine-containing protein. GPX1 is the major isoform of GPX enzyme and is ubiquitously present intracellularly.

Note: At the reported pH optimum of 8.8, we have found the activity to be approx. 10 times that at pH 7.0. However, to remain consistent with literature and avoid complications arising from non-enzymatic oxidation of GSH, our unit is defined at pH 7.0.

Application

Glutathione peroxidase (GPX) from human erythrocytes has been used for the determination of the ability of peroxides to inactivate GPX in epithelial sheets obtained from cutaneous squamous cell carcinoma (CSCC) or solar keratoses (SK).

Biochem/physiol Actions

GPX1 (glutathione peroxidase 1) catalyzes the conversion of multiple organic and inorganic peroxides, by utilizing reduced glutathione (GSH) as an electron donor. This enzyme is also responsible for the removal of organic and inorganic peroxides.

Physical form

Lyophilized powder containing 25% sucrose, 3% dithiothreitol, and sodium phosphate buffer salts

Other Notes

One unit will catalyze the oxidation by H₂O₂ of 1.0 μmole of reduced glutathione to oxidized glutathione per min at pH 7.0 at 25 °C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P304 + P340 - P342 + P311 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable