G3795

Anti-Gravin antibody, Mouse monoclonal

clone JP74, purified from hybridoma cell culture

• Synonym(e): Anti-A-Kinase Anchor Protein 12, Anti-AKAP12
• UNSPSC Code: 12352203
• NACRES: NA.44
• MDL number: MFCD08705585
• Conjugate: unconjugated
• Clone: JP74, monoclonal
• Application: ICC, IP, WB
• Citations: 10

Eigenschaften

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: JP74, monoclonal
• form: buffered aqueous solution
• species reactivity: mouse, rat, human
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.02-0.04 μg/mL using rat brain cytosolic S1 fraction
• isotype: IgG2a
• UniProt accession no.: Q02952
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... AKAP12(9590) ; mouse ... Akap12(83397)

Beschreibung

General description

Monoclonal Anti-Gravin (mouse IgG2a isotype) is derived from the hybridoma JP74 produced by the fusion of mouse myeloma cells (X63Ag8.653) and splenocytes from BALB/c mice immunized with fractionated membrane cortex proteins from human neurons [cell lines NT2-N and SK-N-BE(2)]. Gravin protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain.

Immunogen

fractionated membrane cortex proteins from human neuron cell lines NT2-N and SK-N-BE(2).

Application

Anti-Gravin antibody has been used in western blotting immunofluorescence.

Monoclonal Anti-Gravin antibody is suitable for use in immunoblotting (using mpkCCD cells ). The product may also be used for immunocytochemistry and immunoprecipitation.

Biochem/physiol Actions

Gravin (or AKAP12) is a scaffolding kinase that functions as a tumor suppressor. This kinase interacts with PKC, PKA and other cell cycle signaling proteins to modulate cell division, growth, differentiation and inflammatory responses. The expression of gravin isoforms (α, β, and γ) is regulated by tissue-specific promoters. Gravin also acts as an autoantigen in myasthenia gravis patients .

Monoclonal Anti-Gravin antibody is specific for human, rat and mouse gravin (approx. 300 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)