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FA20

Fructose-Assay-Kit

sufficient for 20 assays

Synonym(e):

Fructose-Testsatz

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PackungsgrößeSKUVerfügbarkeitPreis
1 kit
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€ 196,00

Über diesen Artikel

UNSPSC Code:
12164500
EC Number:
200-333-3
NACRES:
NA.84
Technique(s):
photometry: suitable
Usage:
sufficient for 20 assays
Storage temp.:
2-8°C

€ 196,00


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usage

sufficient for 20 assays

Quality Level

analyte chemical class(es)

sugars (fructose)

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

Application

Dieses Kit dient der quantitativen enzymatischen Bestimmung von Frucose in Lebensmitteln oder anderen Proben. Fructose wird bei der Hexokinase-katalysierten Reaktion durch Adenosintriphosphat (ATP) phosporyliert. Fructose-6-phosphat wird durch Phosphoglucoseisomerase (PGI) zu Glucose-6-phosphat umgewandelt. Glucose-6-phosphat (G6P) wird anschliessend in Gegenwart von Nicotinamidadenindinucleotid (NAD) zu 6-Phosphogluconat oxidiert, wobei Glucose-6-phosphat-Dehydrogenase (G6PDH) als Katalysator fungiert. Während dieser Oxidation wird eine equimolare Menge von NAD zu NADH reduziert. Der damit einhergehende Anstieg der Absorption bei 340 nm ist direkt proportional zur anfänglichen Fruktose-Konzentration in der Probe.

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Dieser Artikel
GAHK20TDF100ASA20
usage

sufficient for 20 assays

usage

sufficient for 20 assays

usage

sufficient for ~100 assays

usage

sufficient for 20 assays

technique(s)

photometry: suitable

technique(s)

photometry: suitable

technique(s)

photometry: suitable

technique(s)

photometry: suitable

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

analyte chemical class(es)

sugars (fructose)

analyte chemical class(es)

sugars (glucose)

analyte chemical class(es)

-

analyte chemical class(es)

sugars

application(s)

food and beverages
general analytical

application(s)

food and beverages
general analytical

application(s)

food and beverages
general analytical

application(s)

food and beverages
general analytical


Nur Kit-Komponenten

Produkt-Nr.
Beschreibung

  • fructose standard 500 mg

  • glucose assay reagent 1 mL/vial

  • phosphoglucose isomerase 1 mL/vial

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

wgk

WGK 3

Lagerklasse

11 - Combustible Solids



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Verwandter Inhalt


Official Methods of Analysis of the AOAC, 28-28 (1995)
Chih-Yu Cheng et al.
FEBS letters, 589(20 Pt B), 3107-3112 (2015-09-04)
Laccase and borate-fructose complex were investigated by coincidence in a solid-state fermentation of Edenia sp. TS-76 under fructose oxidase screening. Laccase was purified to homogeneity with a 34-fold purification and 32% yield. Fructose had no significant effect on laccase activity
Beuter, H.O.
Methods of Enzymatic Analysis, 6, 321-321 (1985)



Global Trade Item Number

SKUGTIN
FA20-1KT04061833622292
G3293-50ML04061837904998
G3293-20ML04061837904981
F2793-500MG04061833613313

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. イネの根および葉のフルクトース含量を測定したいと思っています。こちらのキットを使えるでしょうか。また、商品の詳細(分析手順等)について教えていただけると幸いです。

    1 answer
    1. Yes, this kit would be suitable. Here is the link to the Technical Bulletin that describes the use of this product:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/111/939/fa20bul.pdf
      For roots and leaves, one would follow the Sample Preparation protocol for Solids. The plant tissue would need to be macerated in order to completely extract the Fructose into the water.

      Helpful?

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