Testing for bovine IgG and BSA is not conducted in the final product. A purity agarose gel is performed on F2379, but quantification, testing, or reporting for IgG and BSA is not done. Additionally, the marker size used for QC testing is uncertain, and both IgG and BSA are larger than fetuin.
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Fortfahren mit
biological source
bovine serum (fetal)
form
lyophilized powder
technique(s)
cell culture | mammalian: suitable
impurities
≤0.3% free N-acetylneuraminic acid
solubility
H2O: 1 mg/mL, colorless to faint yellow or tan
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
bovine ... FETUA(504615)
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Dieser Artikel | |||
|---|---|---|---|
| biological source bovine serum (fetal) | biological source bovine serum (fetal) | biological source - | biological source bovine serum |
| technique(s) cell culture | mammalian: suitable | technique(s) HPLC: suitable, MALDI-MS: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized solid |
| impurities ≤0.3% free N-acetylneuraminic acid | impurities ~0.2% free N-acetylneuraminic acid | impurities ≤0.3% free N-acetylneuraminic acid | impurities - |
| solubility H2O: 1 mg/mL, colorless to faint yellow or tan | solubility - | solubility H2O: 1 mg/mL | solubility water: 50 mg/mL |
| Gene Information bovine ... FETUB(504615) | Gene Information bovine ... FETUB(504615) | Gene Information - | Gene Information bovine ... AHSG(280988), FETUB(504615) |
General description
Application
- zum Synthetisieren der primären und sekundären Calciproteinteilchen (CPP)
- zur Untersuchung der Bindung von Xerocomus chrysenteron-Lectin (XCL) an das von Membranproteinen exprimierte T-Antigen
- als Ergänzung in Ham′s F10-Medien für die Kultivierung von Myoblasten.
Biochem/physiol Actions
Preparation Note
Lagerklasse
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Verwandter Inhalt
Product Information Sheet
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Is there information available on the content/concentration of bovine IgG and BSA in the fetuin products F2379 and F3385 (CAS 9014-81-7)?
1 answer-
Helpful?
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How pure is this fetuin? There's a protein content % but could there be other proteins from serum present when using ammonium sulfate fractionation?
1 answer-
Product F2379 can not be considered pure Fetuin. Neither the purity, nor the identity of potential contaminating proteins, is determined.
Helpful?
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Is there a good antibody to recognize this bovine fetuin B by western blot?
1 answer-
Unfortunately, the Fetuin antibodies currently available are not tested against bovine. While fetuin is fairly conserved from bovine to human, reactivity cannot be predicted. See the links below for fetuin antibodies against human that have been testing for use in western blot.
ABN1469
https://www.sigmaaldrich.com/product/mm/abn1469SAB5701234
https://www.sigmaaldrich.com/product/sigma/sab5701234Helpful?
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