E7908
p3XFLAG-CMV™-14 Expression Vector
shuttle vector for transient or stable intracellular expression of C-terminal 3xFLAG
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UNSPSC Code:
12352200
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Unterstützung erhaltenProduktname
p3XFLAG-CMV™-14 Expression Vector, shuttle vector for transient or stable intracellular expression of C-terminal 3xFLAG
tag
3X FLAG tagged
grade
Molecular Biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
Biochem/physiol Actions
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs
General description
The p3XFLAG-CMV™-14 Expression Vector is a 6.3 kb derivative of pCMV51 used to establish expression of transient or stable intracellular C-terminal 3XFLAG™ fusion proteins in mammalian cells. The vector encodes three adjacent FLAG® epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) downstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. Efficiency of replication and genomic integration is optimal when using an SV40 T antigen-expressing host.
The p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.
Vector Maps and Sequences
The p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.
Vector Maps and Sequences
Legal Information
This product is covered by the following patents owned by Sigma-Aldrich Co. LLC: US6,379,903, US7,094,548, JP4405125,EP1220933, CA2386471 and AU774216.
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
Other Notes
- p3XFLAG-CMV™-14 Expression Vector 20 μg (E4901) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- p3XFLAG-CMV™-7-BAP Control Plasmid 20 μg (C7472) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Browse additional application references in our FLAG® Literature portal.
Lagerklasse
10 - Combustible liquids
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Verwandter Inhalt
Instructions
p3xFLAG-CMV-13 and p3xFLAG-CMV-14 Vector Maps
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