E0162

Carboxylesterase 1 isoform c human

recombinant, expressed in baculovirus infected BTI insect cells

• Synonym(e): Carboxylesterase 1 human, carboxylesterase, esterase
• UNSPSC Code: 12352204
• EC Number: 232-773-7
• NACRES: NA.54
• EG-Nummer: 3.1.1.1 (BRENDA, IUBMB)
• Recombinant: expressed in baculovirus infected BTI insect cells
• Concentration: ≥0.3 mg/mL

Eigenschaften

• recombinant: expressed in baculovirus infected BTI insect cells
• Quality Segment: 200
• form: liquid
• concentration: ≥0.3 mg/mL
• shipped in: dry ice
• storage temp.: −70°C

Beschreibung

General description

Carboxylesterase 1 (CE1) is a member of a large multigene[1] carboxylesterase α,β-hydrolase family.[2] It is majorly expressed in the liver.[2] CE1 comprises an αβ domain, a central catalytic domain and a regulatory domain.[2]

This product is offered in a volume of 0.5 mL.

Application

Carboxylesterase 1 isoform c human has been used as a reference standard in carboxylesterase activity from the mussel for comparison of substrate specificity and inhibition studies.[3] It has also been used as a commercial recombinant protein for the methodological validation of environmental chemical-based inhibition studies.[4]

Biochem/physiol Actions

Carboxylesterase enzymes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin.[2] They also hydrolyze long-chain fatty acid esters and thioesters. Carboxylesterase 1 (CE1) catalyzes the formation of cholesteryl esters from cholesterol and fatty acids. Through a transesterification reaction, CE1 also mediates the generation of fatty acid ethyl esters (FAEEs).[2] It also hydrolyzes aromatic and aliphatic esters with preference to small alcohol groups and bulky acyl groups.[5] CE1 metabolizes drug esters and amides carbamates. It participates in the detoxification of environmental toxicants and carcinogens and is useful in pharmacokinetic studies for evaluating pro-drugs.[6]

Other Notes

One unit will hydrolyze one nanomole of 4-nitrophenyl acetate per minute at pH 7.4 at 37 °C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable