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DNTPCA2

Sigma-Aldrich

CleanAmp dNTP

Modified dNTP set for hot-start PCR, 2 μmol of each dNTP

Synonym(e):

Deoxynucleotides, dNTP, dNTP Mix

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About This Item

UNSPSC-Code:
41106305

Form

liquid

Qualität

2 μmol of each dNTP

Leistungsmerkmale

hotstart

Konzentration

50 mM (each dNTP)

Farbe

colorless

Versandbedingung

dry ice

Lagertemp.

−20°C

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Allgemeine Beschreibung

CleanAmp dNTPs, are a product of TriLink BioTechnologies, Inc. CleanAmp dNTPs are modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation. CleanAmp dNTPs are activated by the initial heating step and subsequent denaturing steps in typical hot start PCR cycling conditions. This process limits the amount of activated dNTPs during each cycle of PCR, allowing for more specific and efficient amplification of the desired product and also reducing or completely avoiding mis-priming or primer dimer formation. CleanAmp dNTPs provide a more affordable solution than hot start enzymes for a variety of PCR-based applications.

Anwendung

  • For PCR amplifications that require reduced non-specific amplification
  • For multiplex PCR
  • For reduction of primer dimers
  • Substitute for dNTPs in any PCR reaction

Verpackung

2 μmoles: 4 vials:
40 μL dATP at 50 mM in buffered glycine solution
40 μL dCTP at 50 mM in buffered glycine solution
40 μL dTTP at 50 mM in buffered glycine solution
40 μL dGTP at 50 mM in buffered glycine solution

Rechtliche Hinweise

CleanAmp dNTPs are provided to buyer with a non-transferrable right from TriLink BioTechnologies, Inc. to use the purchased CleanAmp dNTPs in internal research conducted by the buyer, whether the buyer is an academic, non-profit, or for-profit entity. CleanAmp dNTPs are not to be used for human therapeutic or commercial clinical diagnostic use. A license is required for any commercial use of CleanAmp dNTPs, regardless of the academic or non-profit status of the using entity. Information about commercial licenses for CleanAmp dNTPs may be obtained from TriLink BioTechnologies, Inc. Buyer may not use the CleanAmp dNTPs to support the filing of a patent application in any country in the world that contains claims directed to CleanAmp dNTPs or uses thereof without the express approval of TriLink BioTechnologies, Inc. Buyer′s right to have and use the CleanAmp dNTPs will terminate immediately if you fail to comply with these terms and conditions of this agreement.
CleanAmp is a trademark of TriLink BioTechnologies, Inc.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Hot Start activation approaches are increasingly being used to improve the performance of PCR. Since the inception of Hot Start as a means of blocking DNA polymerase extension at lower temperatures, a number of approaches have been developed that target
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Current protocols in molecular biology, Chapter 15, Unit 15-Unit 15 (2009-10-10)
Hot-start PCR is a technique that improves PCR performance by reducing nonspecific amplification during the initial setup stages of the PCR. This unit describes hot-start PCR protocols which utilize primers containing temperature-sensitive modifications. The introduction of 4-oxo-tetradecyl (OXT) phosphotriester groups

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