DNB600

GenElute^™ Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit

• NACRES: NA.52
• UNSPSC Code: 41105600

Eigenschaften

• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: room temp

Beschreibung

Application

• PCR
• qPCR
• Southern Blot
• Methylation-sensitive PCR
• CpG array
• Restriction enzyme digestions
• Viral Detection
• Bacterial Detection
• Microarray
• NGS

General description

Sigma′s GenElute^™ Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit provides a fast, reliable and simple procedures for isolating cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 4 mL. Purification is based on spin column chromatography that uses Sigma′s proprietary resin separation matrix. The kits are designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples. Moreover, these kits allow the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 μL to 50 μL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

• Isolate all sizes of circulating DNA from plasma and serum samples
• Isolate viral and bacterial DNA
• Versatile plasma and serum input volumes (1 mL – 4 mL)
• Concentrate circulating DNA into a flexible elution volume ranging from (50 μL - 100 μL)
• Isolate inhibitor-free cell-free circulating DNA
• Purify high-quality DNA in 40-45 minutes
• Compatible with Streck Cell-Free DNA BCT Tubes

Other Notes

Preps

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H302 + H332,H315,H319,H334
• pcodes: P261 - P264 - P271 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3