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D4540

Dimethylsulfoxid

≥99.5% (GC), suitable for plant cell culture

Dimethyl sulfoxide
1 of 2 reviewers received a sample product or took part in a promotion

Synonym(e):

DMSO, Methylsulfoxid

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Ihnen/SKUVerfügbarkeitPreis
100 mL
Warenkorb auf Verfügbarkeit prüfen
€ 60,40
500 mL
Warenkorb auf Verfügbarkeit prüfen
€ 141,00
1 L
Warenkorb auf Verfügbarkeit prüfen
€ 216,00

Über diesen Artikel

Lineare Formel:
(CH3)2SO
CAS-Nummer:
67-68-5
Molekulargewicht:
78.13
UNSPSC Code:
12191502
NACRES:
NA.71
PubChem Substance ID:
24893881
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
MFCD00002089
Assay:
≥99.5% (GC)
Technique(s):
cell culture | plant: suitable, cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)

€ 60,40

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Technischer Dienst
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Unterstützung erhalten

Quality Segment

200

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

assay

≥99.5% (GC)

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cell culture | plant: suitable, cryopreservation: suitable

impurities

<0.1% water (Karl Fischer)

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Application

Dimethylsulfoxid wurde verwendet:
  • bei der Herstellung von Bisphenol A (BPA)
  • im Zellproliferationsassay (MTT-Assay)
  • im Chemosensitivitätsassay
  • als Vehikelkontrolle in allen Versuchen wie dem Apoptoseassay
  • im Ethoxyresorufin-O-Deethylase(EROD)-Assay

Biochem/physiol Actions

DMSO (Dimethylsulfoxid), ein organisches Lösungsmittel, besitzt verschiedene pharmazeutische Wirkungen wie analgetisch, diuretisch, bakteriostatisch, membranpenetrierend, antiinflammatorisch, vasodilatorisch und cholinesterasehemmend. Es ist ein Nebenprodukt der Holzindustrie und wird als industrielles Lösungsmittel eingesetzt. DMSO wird auch als Therapeutikum zur Behandlung von interstitieller Cystitis und Arthritis eingesetzt.

Other Notes

Gewinner des CiteAb Award 2024 in der Kategorie „Supplier Succeeding in Parkinson′s Research“ (Erfolgreiche Anbieter in der Parkinson-Forschung)

Disclaimer

Unterkühlt leicht und schmilzt langsam bei Raumtemperatur. Das verfestigte Produkt kann durch Erwärmen auf Raumtemperatur wieder verflüssigt werden, ohne dabei geschädigt zu werden.

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Dieser Artikel
D2650D265301934
Dimethylsulfoxid &#8805;99.5% (GC), suitable for plant cell culture

Sigma-Aldrich

D4540

Dimethylsulfoxid

Dimethylsulfoxid Hybri-Max&#8482;, sterile-filtered, BioReagent, suitable for hybridoma, &#8805;99.7%

Sigma-Aldrich

D2650

Dimethylsulfoxid

Dimethylsulfoxid Hybri-Max&#8482;, 0.2 &#956;m filtered, BioReagent, suitable for hybridoma, &#8805;99.7%, Ampoule

Sigma-Aldrich

D2653

Dimethylsulfoxid

Dimethylsulfoxid for inorganic trace analysis, &#8805;99.99995% (metals basis)

Supelco

01934

Dimethylsulfoxid

assay

≥99.5% (GC)

assay

≥99.7%

assay

≥99.7%

assay

≥99.9% (GC), ≥99.99995% (metals basis)

Quality Level

200

Quality Level

400

Quality Level

300

Quality Level

100

technique(s)

cell culture | plant: suitable, cryopreservation: suitable

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

technique(s)

-

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

form

liquid

form

liquid

form

liquid

form

liquid

mp

16-19 °C (lit.)

mp

16-19 °C (lit.)

mp

16-19 °C (lit.)

mp

16-19 °C

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Lagerklasse

10 - Combustible liquids

wgk

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Analysenzertifikate (COA)

Lot/Batch Number

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2 Reviews This action will navigate to reviews.

Questions

1–10 of 11 Questions  

  1. Can I use this product (suitable for plant cell culture) for cyropreservation of mammalian cells? Does cryopreservation of mammalian cells require sterile-filtered DMSO?

    1 answer

    1. D4540 is listed as suitable for cryopreservation.
      However, D2650 is already pre-sterile and has documentation with a protocol for cryopreservation of human and animal cell lines.

      See these links:
      https://www.sigmaaldrich.com/product/sigma/d2650
      https://www.sigmaaldrich.com/product/sigma/d2650#product-documentation

      Look for the Product Information Sheet. Also, see this link for more information:
      https://www.sigmaaldrich.com/products/cell-culture-and-analysis/cell-culture-supplements-and-reagents/cell-freezing-reagents

      Helpful?

  2. Can I autoclave this product?

    1 answer

    1. DMSO is not autoclavable in neat form. It is highly unlikely that microorganisms would survive in this environment. Should sterilization be necessary a PTFE, Polypropylene, or Nylon filter membrane are recommended for sterile-filtration.

      Helpful?

  3. What is the molarity of DMSO?

    1 answer

    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      Helpful?

  4. Does DMSO have any synthetic utility?

    1 answer

    1. DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

      Helpful?

  5. What is the DMSO dissolved in?

    1 answer

    1. DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

      Helpful?

  6. Where does DMSO come from?

    1 answer

    1. DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

      Helpful?

  7. What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?

    1 answer

    1. DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. Is Dimethyl sulfoxide a sterile product?

    1 answer

    1. No, this product is not considered sterile.  Product Nos. D2560 and D2438 are sterile-filtered.

      Helpful?

  10. What concentration of DMSO do I use in a cell freezing media?

    1 answer

    1. DMSO is usually used at 1-10%, depending on the cell line.  For the cell freezing media that we offer, it is used at 8.7% concentration.

      Helpful?

1–10 of 11 Questions  
2 Reviews This action will navigate to reviews.
1 of 2 reviewers received a sample product or took part in a promotion
1 out of 1 (100%) reviewers recommend this product

Reviews

Active Filters

  1. San Diego
    • Review 1
    • Votes 4
    5 out of 5 stars.

    Works on mammalian cells

    Good for mammalian cell culture treatments of compound

    Helpful?

  2. 1 Ratings-only review