C0844

94090167

• UNSPSC-Code: 12352200

Eigenschaften

• Biologische Quelle: human blood
• Nummer des HRC-Gremiums: Not specified
• Wachstumsmodus: Suspension
• Karyotyp: Not Specified
• Morphologie: Lymphoblastoid

Beschreibung

Ursprung der Zelllinie

UK Caucasian Male

Beschreibung der Zelllinie

A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.

This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.

Anwendung

As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.

Nährmedium

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 20% FBS / FCS (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subkultur-Routine

Maintain cultures between 3 x 10⁵ - 2 x 10⁶ cells/ml; 5% CO₂, 37 °C.

Sonstige Hinweise

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.