M3 Clone M-3

93120827, mouse skin, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: M3 Clone M-3, 93120827
• Biologische Quelle: mouse skin
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 83; hypertetraploid
• Morphologie: Epithelial
• Produkte: Melanin
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse Cloudman S 91 melanoma

Beschreibung der Zelllinie

Melanin producing cell line, derived from a Cloudman S 91 melanoma induced in a F1 (CxDBA) male mouse. The line is tumourigenic in mice and susceptible to a variety of viruses including herpes simplex, vaccinia, pseudorabies and vesicular stomatitis virus (Indiana virus).

Anwendung

Tumourigenicity studies

Nährmedium

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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